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Product Info Summary
| SKU: | A07959 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-PLEKHM1 Antibody
SKU/Catalog Number
A07959
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PLEKHM1 Antibody (Catalog # A07959). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
PLEKHM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PLEKHM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07959)
Host
Rabbit
Contents
PLEKHM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PLEKHM1 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human PLEKHM1. The immunogen is located within amino acids 50 - 100 of PLEKHM1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Multiple isoforms of CAPS2 are known to exist. This CAPS2 antibody is predicted to be specific to CAPS2 and not recognize CAPS1.
Reactive Species
A07959 is reactive to PLEKHM1 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
117443 MW
Background of PLEKHM1
PLEKHM1 is a member of the M family of Pleckstrin homolog domain-containing proteins, a group of proteins containing a RUN domain, two pleckstrin homology domains, and a cysteine-rich domain. It was identified through segregation analysis as a cause of osteopetrosis in humans. PLEKHM1 co-localizes with Rab7 to late endosomal/lysosomal vesicles in HEK293 and osteoclast-like cells, with this co-localization dependent on the prenylation of Rab7. Monocytes from a patient homozygous for a mutated form of PLEKHM1differentiated into osteoclasts normally, but failed to form ruffled borders and showed little evidence of bone resorbtion when cultured on dentine discs. Another mutation of PLEKHM1 impaired vesicular acidification and increased TRACP secretion in osteoclasts, suggesting that PLEKHM1 has critical roles in endosomal maturation and may be important in osteoclast-osteoblast cross-talk.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07959 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PLEKHM1 antibody can be used for detection of PLEKHM1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PLEKHM1 in human lung tissue lysate with PLEKHM1 antibody at 1 μg/mL.
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Immunohistochemistry of PLEKHM1 in human lung tissue with PLEKHM1 antibody at 5 μg/mL.
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Immunofluorescence of PLKHM1 in human lung tissue with PLKHM1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For PLEKHM1 (Source: Uniprot.org, NCBI)
Gene Name
PLEKHM1
Full Name
Pleckstrin homology domain-containing family M member 1
Weight
117443 MW
Alternative Names
B2, AP162, OPTB6, KIAA0356, Pleckstrin homology domain-containing family M member 1, 162 kDa adapter protein, PH domain-containing family M member 1 PLEKHM1 AP162, B2, OPTA3, OPTB6 pleckstrin homology and RUN domain containing M1 pleckstrin homology domain-containing family M member 1|162 kDa adapter protein|PH domain-containing family M member 1|pleckstrin homology domain containing, family M (with RUN domain) member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLEKHM1, check out the PLEKHM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLEKHM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PLEKHM1 Antibody (A07959)
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