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Product Info Summary
| SKU: | A02146-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Plakophilin 2/PKP2 Antibody Picoband®
View all Plakophilin 2 Antibodies
SKU/Catalog Number
A02146-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Plakophilin 2/PKP2 Antibody Picoband® catalog # A02146-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Plakophilin 2/PKP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02146-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Plakophilin 2/PKP2 recombinant protein (Position: E9-D866).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02146-1 is reactive to PKP2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
92 kDa
Calculated molecular weight
97.415kDa
Background of Plakophilin 2
Plakophilin-2 is a protein that in humans is encoded by the PKP2 gene. This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This gene may regulate the signaling activity of beta-catenin and is required to maintain transcription of genes that control intracellular calcium cycling including ryanodine receptor 2, ankyrin-B, triadin, and calcium channel, voltage-dependent, L type, alpha 1C. Mutations in this gene are associated with different inherited cardiac conditions including Arrythmogenic Cardiomyopathy, Brugada Syndrome, and Idiopathic Ventricular Fibrillation. A processed pseudogene with high similarity to this gene has been mapped to chromosome 12p13.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02146-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human Hela whole cell, rat heart tissue, mouse heart tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Plakophilin 2/PKP2 using anti-Plakophilin 2/PKP2 antibody (A02146-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat heart tissue lysates,
Lane 4: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plakophilin 2/PKP2 antigen affinity purified polyclonal antibody (Catalog # A02146-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plakophilin 2/PKP2 at approximately 92 kDa. The expected band size for Plakophilin 2/PKP2 is at 97 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PKP2 (Source: Uniprot.org, NCBI)
Gene Name
PKP2
Full Name
Plakophilin-2
Weight
97.415kDa
Superfamily
beta-catenin family
Alternative Names
Mucin-6; MUC-6; Gastric mucin-6; MUC6 PKP2 ARVD9 plakophilin 2 plakophilin-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PKP2, check out the PKP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PKP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Plakophilin 2/PKP2 Antibody Picoband® (A02146-1)
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