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Product Info Summary
| SKU: | M01391-2 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-TFF1 Antibody (Center)
SKU/Catalog Number
M01391-2
Size
50 µl
Description
Boster Bio Anti-TFF1 Antibody (Center) (Catalog # M01391-2). Tested in WB, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-TFF1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01391-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This TFF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 11-40 amino acids from the Central region of human TFF1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01391-2 is reactive to TFF1 in Human
Applications
M01391-2 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
9150 Da
Background of TFF1/pS2
Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling TFF1 with M01391-2 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing secreted staining on MCF-7 cell line. The nuclear counter stain is DAPI (blue).
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All lanes : Anti-TFF1 Antibody (Center) at 1:2000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: T47D whole cell lysate
Lane 3: SK-BR-3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 9 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For TFF1 (Source: Uniprot.org, NCBI)
Gene Name
TFF1
Full Name
Trefoil factor 1
Weight
9150 Da
Alternative Names
BCEI; BCEIbreast cancer, estrogen-inducible sequence expressed in; Breast cancer estrogen-inducible protein; breast cancer estrogen-inducible sequence; D21S21; gastrointestinal trefoil protein pS2; hP1.A; HPS2; PNR-2; Polypeptide P1.A; Protein pS2; PS2; TFF1; trefoil factor 1; trefoil factor, BCE1, human pS2 induced by estrogen from human breast cancercell line M10HP1.A TFF1 BCEI, D21S21, HP1.A, HPS2, pNR-2, pS2 trefoil factor 1 trefoil factor 1|breast cancer estrogen-inducible protein|breast cancer estrogen-inducible sequence|gastrointestinal trefoil protein pS2|polypeptide P1.A|protein pS2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TFF1, check out the TFF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TFF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TFF1 Antibody (Center) (M01391-2)
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