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Product Info Summary
| SKU: | A12582-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-PKIB Antibody Picoband™
SKU/Catalog Number
A12582-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PKIB Antibody Picoband™ catalog # A12582-3. Tested in ELISA, IHC applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PKIB Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12582-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PKIB recombinant protein (Position: M1-K78).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A12582-3 is reactive to PKIB in Human
Applications
A12582-3 is guaranteed for ELISA, IHC Boster Guarantee
Observed Molecular Weight
110 kDa
Calculated molecular weight
Background of PKI-beta
cAMP-dependent protein kinase inhibitor beta is a protein that in humans is encoded by the PKIB gene. This gene encodes a member of the cAMP-dependent protein kinase inhibitor family. The encoded protein may play a role in the protein kinase A (PKA) pathway by interacting with the catalytic subunit of PKA, and overexpression of this gene may play a role in prostate cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. IHC analysis of PKIB using anti-PKIB antibody (A12582-3).
PKIB was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PKIB Antibody (A12582-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PKIB (Source: Uniprot.org, NCBI)
Gene Name
PKIB
Full Name
cAMP-dependent protein kinase inhibitor beta
Weight
Superfamily
PKI family
Alternative Names
cAMP-dependent protein kinase inhibitor 2; cAMP-dependent protein kinase inhibitor beta; FLJ23817; PKI-beta; PRKACN2; protein kinase (cAMP-dependent, catalytic) inhibitor beta PKIB PRKACN2 cAMP-dependent protein kinase inhibitor beta cAMP-dependent protein kinase inhibitor beta|PKI-beta|cAMP-dependent protein kinase inhibitor 2|protein kinase (cAMP-dependent, catalytic) inhibitor beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PKIB, check out the PKIB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PKIB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKIB Antibody Picoband™ (A12582-3)
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