Anti-mtTFA/Tfam Antibody Picoband™

Product Name

Anti-mtTFA/Tfam Antibody Picoband™

View all mtTFA Antibodies

SKU/Catalog Number

A01119-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-mtTFA/Tfam Antibody Picoband™ catalog # A01119-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-mtTFA/Tfam Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01119-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse mtTFA/Tfam recombinant protein (Position: M1-D211).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01119-1 is reactive to TFAM in Mouse, Rat

Applications

A01119-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

23 kDa

Calculated molecular weight

Background of mtTFA

TFAM (Transcription factor A, mitochondrial), also known as TCF6 or TCF6L2, is a 162-amino acid protein that activates transcription of each mitochondrial DNA (mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al. (1992) mapped TFAM sequences, which they called MTTF1, to 3 different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott (2007) stated that the sequences mapped to chromosomes 7p (TCF6L1) and 11q (MTTF1, or TCF6L3) are pseudogenes. Larsson et al. (1997) mapped the mouse mitochondrial transcription factor A gene (Tfam) to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of mtTFA/Tfam using anti-mtTFA/Tfam antibody (A01119-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: rat PC-12 whole cell lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse spleen tissue lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-mtTFA/Tfam antigen affinity purified polyclonal antibody (Catalog # A01119-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for mtTFA/Tfam at approximately 23KD. The expected band size for mtTFA/Tfam is at 23KD.

Gene/Protein Information For TFAM (Source: Uniprot.org, NCBI)

Alternative Names

mitochondrial transcription factor A; MtTF1; mtTFA; TCF-6; TCF6L1; TCF6L2Mitochondrial transcription factor 1; TCF6TCF6L3; Transcription factor 6; Transcription factor 6-like 2 (mitochondrial transcription factor); Transcription factor 6-like 2; transcription factor A, mitochondrial TFAM MTDPS15, MTTF1, MTTFA, TCF6, TCF6L1, TCF6L2, TCF6L3 transcription factor A, mitochondrial transcription factor A, mitochondrial|mitochondrial transcription factor 1|mitochondrial transcription factor A|transcription factor 6|transcription factor 6-like 1|transcription factor 6-like 2 (mitochondrial transcription factor)|transcription factor 6-like 3

For more info on TFAM, check out the TFAM Infographic

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In this infographic, you will see the following information for TFAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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