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Product Info Summary
| SKU: | M12461 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NDUFB3 Antibody (N-Term)
SKU/Catalog Number
M12461
Size
50 µl
Description
Boster Bio Anti-NDUFB3 Antibody (N-Term) (Catalog # M12461). Tested in WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-NDUFB3 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12461)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This NDUFB3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 13-45 amino acids from human NDUFB3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M12461 is reactive to NDUFB3 in Human, Mouse
Reconstitution
Calculated molecular weight
11402 Da
Background of NDUFB3
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M12461 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-NDUFB3 Antibody (N-Term) at 1:8000 dilution
Lane 1: human heart lysate
Lane 2: mouse brain lysate
Lane 3: mouse heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 11 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-NDUFB3 Antibody (N-Term) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: A431 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 11kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For NDUFB3 (Source: Uniprot.org, NCBI)
Gene Name
NDUFB3
Full Name
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
Weight
11402 Da
Superfamily
complex I NDUFB3 subunit family
Alternative Names
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3, Complex I-B12, CI-B12, NADH-ubiquinone oxidoreductase B12 subunit, NDUFB3 NDUFB3 B12, CI-B12, MC1DN25 NADH:ubiquinone oxidoreductase subunit B3 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3|NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 3, 12kDa|NADH-ubiquinone oxidoreductase B12 subunit|complex I-B12
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NDUFB3, check out the NDUFB3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NDUFB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NDUFB3 Antibody (N-Term) (M12461)
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