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Product Info Summary
| SKU: | A00822Y52 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PKC Delta (phospho-Y52) PRKCD Antibody
SKU/Catalog Number
A00822Y52
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PKC Delta (phospho-Y52) PRKCD Antibody catalog # A00822Y52. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKC Delta (phospho-Y52) PRKCD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00822Y52)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human PKC δ around the phosphorylation site of Tyrosine 52.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00822Y52 is reactive to PRKCD in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
77505 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00822Y52 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of PRKCD using Anti-PKC Delta (phospho-Y52) PRKCD Antibody (A00822Y52).
Immunohistochemistry (IHC) analyzes of p-PKC δ (Y52) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody
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Figure 1. Western blotting validation for Anti-PKC Delta (phospho-Y52) PRKCD Antibody A00822Y52
Western blot (WB) analysis of p-PKC δ (Y52) polyclonal antibody at 1:500 dilutionLane1:MCF-7 cell lysate treated with serum starvation(24h)
Lane2:Raw264.7 cell lysate treated with serum starvation(24h)
Lane3:H9C2 cell lysate treated with serum starvation(24h)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For PRKCD (Source: Uniprot.org, NCBI)
Gene Name
PRKCD
Full Name
Protein kinase C delta type
Weight
77505 MW
Superfamily
protein kinase superfamily
Alternative Names
Protein kinase C delta type;2.7.11.13 PRKCD ALPS3, CVID9, MAY1, PKCD, nPKC-delta protein kinase C delta protein kinase C delta type|protein kinase C delta VIII|tyrosine-protein kinase PRKCD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKCD, check out the PRKCD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKCD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKC Delta (phospho-Y52) PRKCD Antibody (A00822Y52)
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