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Product Info Summary
| SKU: | A01070 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-nNOS (neuronal)/NOS1 Antibody Picoband®
SKU/Catalog Number
A01070
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-nNOS (neuronal)/NOS1 Antibody Picoband® catalog # A01070. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-nNOS (neuronal)/NOS1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01070)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human nNOS(neuronal), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01070 is reactive to NOS1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
160 kDa
Calculated molecular weight
160970 MW
Background of nNOS
Nitric oxide synthase 1 (neuronal), also known as NOS1, is an enzyme that in humans is encoded by the NOS1 gene. The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01070 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of nNOS using anti-nNOS antibody (A01070).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: rat brain tissue lysates,
lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-nNOS antigen affinity purified polyclonal antibody (Catalog # A01070) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for nNOS at approximately 160KD. The expected band size for nNOS is at 160KD.
Protein Target Info & Infographic
Gene/Protein Information For NOS1 (Source: Uniprot.org, NCBI)
Gene Name
NOS1
Full Name
Nitric oxide synthase, brain
Weight
160970 MW
Superfamily
NOS family
Alternative Names
Nitric oxide synthase, brain;1.14.13.39;Constitutive NOS;NC-NOS;N NOS1 IHPS1, N-NOS, NC-NOS, NOS, bNOS, nNOS nitric oxide synthase 1 nitric oxide synthase, brain|NOS type I|constitutive NOS|neuronal NOS|nitric oxide synthase 1 (neuronal)|peptidyl-cysteine S-nitrosylase NOS1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NOS1, check out the NOS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NOS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-nNOS (neuronal)/NOS1 Antibody Picoband® (A01070)
Hello CJ!
A01070 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Singh S,Kumar V,Kumar N,Sharma P,Waheed SM.Protective and Modulatory Effects of Trapa bispinosa and Trigonella foenum-graecum on Neuroblastoma Cells Through Neuronal Nitric Oxide Synthase.Assay Drug Dev Technol.2020 Jan;18(1):64-74.doi:10.1089/adt.2018.91
Species: Mouse
A01070 usage in article: APP:WB, SAMPLE:NB-41 CELL, DILUTION:1:1000
George AK, Behera J, Kelly KE, Mondal NK, Richardson KP, Tyagi N. Sci Rep. 2018 Mar 26;8(1):5158. doi: 10.1038/s41598-018-23568-z. Exercise Mitigates Alcohol Induced Endoplasmic Reticulum Stress Mediated Cognitive Impairment through ATF6-Herp Signaling.
Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation
Distribution of constitutive nitric oxide synthase in the jejunum of adult rat
Kong L, Wei Q, Fedail Js, Shi F, Nagaoka K, Watanabe G. J Reprod Dev. 2015;61(3):219-27. Doi: 10.1262/Jrd.2014-129. Epub 2015 Mar 20. Effects Of Thyroid Hormones On The Antioxidative Status In The Uterus Of Young Adult Rats.
Zhang W, Wei Qw, Wang Zc, Ding W, Wang W, Shi Fx. J Zhejiang Univ Sci B. 2011 Jan;12(1):55-64. Doi: 10.1631/Jzus.B1000174. Cell-Specific Expression And Immunolocalization Of Nitric Oxide Synthase Isoforms And The Related Nitric Oxide/Cyclic Gmp Si...
Wu R, Yin J, Yao K, Haefliger I. Mol Vis. 2007 Apr 2;13:493-6. Inhibition By Brimonidine Of Forskolin-Induced Nitric Oxide Synthase Expression In Human Ciliary Bodies In Vitro.
Chang Mj, Xiao Jh, Wang Y, Yan Yl, Yang J, Wang Jl. Plos One. 2012;7(12):E50291. Doi: 10.1371/Journal.Pone.0050291. Epub 2012 Dec 3. 2, 3, 5, 4'-Tetrahydroxystilbene-2-O-Beta-D-Glucoside Improves Gastrointestinal Motility Disorders In Stz-Induced ...
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-nNOS (neuronal)/NOS1 Antibody Picoband®
Question
My question regards using your anti-nNOS (neuronal)/NOS1 antibody for negative regulation of calcium ion transport studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
We appreciate your inquiry. This A01070 anti-nNOS (neuronal)/NOS1 antibody is tested on mouse brain. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-31
Question
Our lab were content with the WB result of your anti-nNOS (neuronal)/NOS1 antibody. However we have observed positive staining in skeletal muscle cell membrane using this antibody. Is that expected? Could you tell me where is NOS1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-04-27
Answer
From what I have seen in literature, skeletal muscle does express NOS1. Generally NOS1 expresses in cell membrane, sarcolemma. Regarding which tissues have NOS1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 7678401
Cerebellum, Pubmed ID: 7515942
Retina, Pubmed ID: 8879752
Skeletal muscle, Pubmed ID: 9791007
Testis, Pubmed ID: 9111048
Boster Scientific Support
Answered: 2018-04-27
Question
We are currently using anti-nNOS (neuronal)/NOS1 antibody A01070 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-19
Answer
The anti-nNOS (neuronal)/NOS1 antibody (A01070) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-19
Question
We have seen staining in mouse vastus lateralis. Are there any suggestions? Is anti-nNOS (neuronal)/NOS1 antibody supposed to stain vastus lateralis positively?
D. Krishna
Verified customer
Asked: 2016-06-03
Answer
From literature vastus lateralis does express NOS1. From Uniprot.org, NOS1 is expressed in vastus lateralis, cerebellum, brain, retina, testis, skeletal muscle, among other tissues. Regarding which tissues have NOS1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 7678401
Cerebellum, Pubmed ID: 7515942
Retina, Pubmed ID: 8879752
Skeletal muscle, Pubmed ID: 9791007
Testis, Pubmed ID: 9111048
Boster Scientific Support
Answered: 2016-06-03
