Product Info Summary
SKU: | P00024-4 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, WB |
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Product info
Product Name
Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00024-4
BM4762 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody catalog # P00024-4. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00024-4)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HIA-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human AKT1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00024-4 is reactive to AKT1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
65 kDa
Calculated molecular weight
55686 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00024-4 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IP 1:50
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Phospho-AKT1 (S124) expression in (1) MCF-7 cell treated with Alkaline Phosphatase lysate; (2) MCF-7 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For AKT1 (Source: Uniprot.org, NCBI)
Gene Name
AKT1
Full Name
RAC-alpha serine/threonine-protein kinase
Weight
55686 MW
Superfamily
protein kinase superfamily
Alternative Names
RAC-alpha serine/threonine-protein kinase;2.7.11.1;Protein kinase B;PKB;Protein kinase B alpha;PKB alpha;Proto-oncogene c-Akt;RAC-PK-alpha;AKT1;PKB, RAC; AKT1 AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA AKT serine/threonine kinase 1 RAC-alpha serine/threonine-protein kinase|AKT1m|PKB alpha|RAC-PK-alpha|protein kinase B alpha|proto-oncogene c-Akt|rac protein kinase alpha|serine-threonine protein kinase|v-akt murine thymoma viral oncogene homolog 1|v-akt murine thymoma viral oncogene-like protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AKT1, check out the AKT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AKT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody (P00024-4)
Hello CJ!
P00024-4 has been cited in 14 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A small interfering RNA targeting osteopontin as gastric cancer therapeutics
Short hairpin RNA targeting AKT1 and PI3K/p85 suppresses the proliferation and self-renewal of lung cancer stem cells
Protective Effects of Sodium (?)-5-Bromo-2-(%u03B1-Hydroxypentyl) Benzoate in a Rodent Model of Global Cerebral Ischemia
Vibration exercise decreases insulin resistance and modulates the insulin signaling pathway in a type 2 diabetic rat model
Epigallocatechin-3-Gallate Promotes the Growth of Mink Hair Follicles Through Sonic Hedgehog and Protein Kinase B Signaling Pathways
Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways
Epicatechin Plus Treadmill Exercise are Neuroprotective Against Moderate-stage Amyloid Precursor Protein/Presenilin 1 Mice
Progesterone alleviates hypoxic-ischemic brain injury via the Akt/GSK-3? signaling pathway
Li R, Cui K, Liu K, Li H, Zhang Y, Liu X, Chen R, Li M, Wang T, Wang S, Liu J, Rao K. Sci Rep. 2017 Oct 18;7(1):13464. doi: 10.1038/s41598-017-12907-1. Metabolic syndrome in rats is associated with erectile dysfunction by impairing PI3K/Akt/eNOS a...
Wang Q, Sun X, Li X, Dong X, Li P, Zhao L. Mol Med Rep. 2015 Jan;11(1):151-8. Doi: 10.3892/Mmr.2014.2762. Epub 2014 Oct 23. Resveratrol Attenuates Intermittent Hypoxia-Induced Insulin Resistance In Rats: Involvement Of Sirtuin 1 And The Phosphatid...
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody
Question
We have been able to see staining in human cervix carcinoma. What should we do? Is anti-Phospho-AKT1 (S124) Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
From literature cervix carcinoma does express AKT1. From Uniprot.org, AKT1 is expressed in left adrenal gland, adrenal gland, muscle ovary, foreskin, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-07-11
Question
Our team were happy with the WB result of your anti-Phospho-AKT1 (S124) Rabbit Monoclonal antibody. However we have been able to see positive staining in left adrenal gland cytoplasm. using this antibody. Is that expected? Could you tell me where is AKT1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-09-27
Answer
Based on literature, left adrenal gland does express AKT1. Generally AKT1 expresses in cytoplasm. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-09-27
Question
We have tried in the past anti-Phospho-AKT1 (S124) Rabbit Monoclonal antibody for WB on foreskin in a previous experiment. I am using mouse, and We want to use the antibody for IP next. I am looking for examining foreskin as well as left adrenal gland in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2018-01-26
Answer
I have checked the website and datasheets of our anti-Phospho-AKT1 (S124) Rabbit Monoclonal antibody and I see that P00024-4 has been tested on mouse in both WB and IP. Thus P00024-4 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-01-26
Question
We are currently using anti-Phospho-AKT1 (S124) Rabbit Monoclonal antibody P00024-4 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
P. Krishna
Verified customer
Asked: 2014-04-18
Answer
The anti-Phospho-AKT1 (S124) Rabbit Monoclonal antibody (P00024-4) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-04-18