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Product Info Summary
| SKU: | A02993 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-Pituitary homeobox 1 Pitx1 Antibody
SKU/Catalog Number
A02993
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Pituitary homeobox 1 Pitx1 Antibody catalog # A02993. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Pituitary homeobox 1 Pitx1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02993)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PITX1. AA range:121-170
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02993 is reactive to PITX1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
34128 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02993 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of Jurkat cells using Pitx1 Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using Pitx1 Polyclonal antibody.
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Western blot analysis of lysates from Jurkat and COLO cells, using PITX1 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For PITX1 (Source: Uniprot.org, NCBI)
Gene Name
PITX1
Full Name
Pituitary homeobox 1
Weight
34128 MW
Superfamily
paired homeobox family
Alternative Names
Pituitary homeobox 1;Hindlimb-expressed homeobox protein backfoot;Homeobox protein PITX1;Paired-like homeodomain transcription factor 1;PITX1;BFT, PTX1; PITX1 BFT, CCF, LBNBG, POTX, PTX1 paired like homeodomain 1 pituitary homeobox 1|hindlimb expressed homeobox protein backfoot|homeobox protein PITX1|paired-like homeodomain transcription factor 1|pituitary homeo box 1|pituitary otx-related factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PITX1, check out the PITX1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PITX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Pituitary homeobox 1 Pitx1 Antibody (A02993)
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No publications found for A02993
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Customer Q&As
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7 Customer Q&As for Anti-Pituitary homeobox 1 Pitx1 Antibody
Question
Is this A02993 anti-Pitx1 antibody reactive to the isotypes of PITX1?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
The immunogen of A02993 anti-Pitx1 antibody is Synthesized peptide derived from the Internal region of human Pitx1. at AA rangle: 90-170. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-26
Question
We are currently using anti-Pitx1 antibody A02993 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
The anti-Pitx1 antibody (A02993) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-22
Question
I see that the anti-Pitx1 antibody A02993 works with WB, what is the protocol used to produce the result images on the product page?
J. Taylor
Verified customer
Asked: 2019-02-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-02-26
Question
Can you help my question with product A02993, anti-Pitx1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-11-02
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02993 anti-Pitx1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-02
Question
I would like to test anti-Pitx1 antibody A02993 on rat lower esophagus mucosa for research purposes, then I may be interested in using anti-Pitx1 antibody A02993 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-07-09
Answer
The products we sell, including anti-Pitx1 antibody A02993, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-09
Question
Is a blocking peptide available for product anti-Pitx1 antibody (A02993)?
D. Patel
Verified customer
Asked: 2016-12-23
Answer
We do provide the blocking peptide for product anti-Pitx1 antibody (A02993). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-12-23
Question
I have attached the WB image, lot number and protocol we used for lower esophagus mucosa using anti-Pitx1 antibody A02993. Please let me know if you require anything else.
E. Roberts
Verified customer
Asked: 2015-01-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-06
