Mouse Plasma Kallikrein/KLKB1 ELISA Kit PicoKine®

Plasma Kallikrein/KLKB1 ELISA kit for Mouse

Mouse KLKB1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Klkb1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: EK2107
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Mouse Plasma Kallikrein/KLKB1 ELISA Kit PicoKine®

View all Plasma Kallikrein/KLKB1 ELISA kits

SKU/Catalog Number

EK2107

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse KLKB1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Klkb1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse Plasma Kallikrein/KLKB1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2107)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: G20-A638

Sensitivity

<50 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK2107 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2107 is reactive to Klkb1 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK2107 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Plasma Kallikrein/KLKB1

Plasma kallikrein is a protein that in humans is encoded by the KLKB1 gene. It is mapped to 4q35.2. This gene encodes a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. The encoded preproprotein present in plasma as a non-covalent complex with high molecular weight kininogen undergoes proteolytic processing mediated by activated coagulation factor XII to generate a disulfide-linked, heterodimeric serine protease comprised of heavy and light chains. Certain mutations in this gene cause prekallikrein deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms.

Kit Components

Catalog Number Description Quantity
EK2107-CAP Anti-Mouse Klkb1 Pre-coated 96-well strip microplate 1
EK2107-ST Mouse Klkb1 Standard 2 vials, 10 ng/tube
EK2107-DA Mouse Klkb1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0230.1510.2880.4920.8111.2421.6112.006

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Plasma Kallikrein/KLKB1, Dilution ratio of 1:2000, concentration in serum and plasma is around 4 ug/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Plasma Kallikrein/KLKB1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2471236480629512454921
Standard deviation11.1270.45221.0820.9585.91314.94
CV (%)4.5%5.7%4.6%7.1%6.9%6.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Plasma Kallikrein/KLKB1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 125227523527225816.196.2%
Sample 21856177616911826178762.483.4%
Sample 342964210383246174238279.566.5%
*number of samples for each test n=16.

Gene/Protein Information For Klkb1 (Source: Uniprot.Org, NCBI)

Gene Name

Klkb1

Full Name

Plasma kallikrein

Weight

71.37kDa

Superfamily

peptidase S1 family

Alternative Names

Plasma kallikrein; Fletcher factor; Kininogenin; Plasma prekallikrein; PKK; Plasma kallikrein heavy chain; Plasma kallikrein light chain; KLKB1; KLK3 KLKB1 KLK3, PKK, PKKD, PPK kallikrein B1 plasma kallikrein|kallikrein B, plasma (Fletcher factor) 1|kininogenin|plasma prekallikrein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Klkb1, check out the Klkb1 Infographic

Klkb1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Klkb1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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3 Customer Q&As for Mouse Plasma Kallikrein/KLKB1 ELISA Kit PicoKine®

Question

Does EK2107 contain native human biological materials?

Verified customer

Asked: 2021-11-10

Answer

The Mouse Plasma Kallikrein/KLKB1 ELISA Kit PicoKine® (EK2107) doesn't contain native human biological materials. doesn't contain native human biological materials.

Boster Scientific Support

Answered: 2021-11-11

Question

Q: is it okay to use heparin plasma as samples in Mouse Plasma Kallikrein Picokine® ELISA Kit (Catalog # EK2107)?

D. White

Verified customer

Asked: 2020-04-04

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Plasma Kallikrein causing degradation of its protein structure. Plasma Kallikrein may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Plasma Kallikrein ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-04-04

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

N. Chakrabarti

Verified customer

Asked: 2016-08-30

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2016-08-30

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