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Product Info Summary
| SKU: | EK2107 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Mouse Plasma Kallikrein/KLKB1 ELISA Kit PicoKine®
View all Plasma Kallikrein/KLKB1 ELISA kits
SKU/Catalog Number
EK2107
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse KLKB1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Klkb1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Plasma Kallikrein/KLKB1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2107)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: G20-A638
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2107 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2107 is reactive to KLKB1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2107 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Plasma Kallikrein/KLKB1
Plasma kallikrein is a protein that in humans is encoded by the KLKB1 gene. It is mapped to 4q35.2. This gene encodes a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. The encoded preproprotein present in plasma as a non-covalent complex with high molecular weight kininogen undergoes proteolytic processing mediated by activated coagulation factor XII to generate a disulfide-linked, heterodimeric serine protease comprised of heavy and light chains. Certain mutations in this gene cause prekallikrein deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2107-CAP | Anti-Mouse KLKB1 Pre-coated 96-well strip microplate | 1 |
| EK2107-ST | Mouse KLKB1 Standard | 2 vials, 10 ng/tube |
| EK2107-DA | Mouse KLKB1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.023 | 0.151 | 0.288 | 0.492 | 0.811 | 1.242 | 1.611 | 2.006 |
Data Example Images
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Mouse KLKB1 PicoKine ELISA Kit
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Plasma Kallikrein/KLKB1, Dilution ratio of 1:2000, concentration in serum and plasma is around 4 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Plasma Kallikrein/KLKB1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 247 | 1236 | 4806 | 295 | 1245 | 4921 |
| Standard deviation | 11.12 | 70.45 | 221.08 | 20.95 | 85.91 | 314.94 |
| CV (%) | 4.5% | 5.7% | 4.6% | 7.1% | 6.9% | 6.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Plasma Kallikrein/KLKB1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 252 | 275 | 235 | 272 | 258 | 16.19 | 6.2% |
| Sample 2 | 1856 | 1776 | 1691 | 1826 | 1787 | 62.48 | 3.4% |
| Sample 3 | 4296 | 4210 | 3832 | 4617 | 4238 | 279.56 | 6.5% |
Protein Target Info & Infographic
Gene/Protein Information For KLKB1 (Source: Uniprot.Org, NCBI)
Gene Name
KLKB1
Full Name
Plasma kallikrein
Weight
Superfamily
peptidase S1 family
Alternative Names
EC 3.4.21; EC 3.4.21.34; Fletcher factor; kallikrein B, plasma (Fletcher factor) 1; kininogenin; KLK3plasma kallikrein; KLKB1; plasma kallikrein heavy chain; plasma kallikrein light chain; Plasma Kallikrein; Plasma Prekallikrein; PPK KLKB1 KLK3, PKK, PKKD, PPK kallikrein B1 plasma kallikrein|kallikrein B, plasma (Fletcher factor) 1|kininogenin|plasma prekallikrein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on KLKB1, check out the KLKB1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for KLKB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Plasma Kallikrein/KLKB1 ELISA Kit PicoKine® (EK2107)
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Customer Q&As
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3 Customer Q&As for Mouse Plasma Kallikrein/KLKB1 ELISA Kit PicoKine®
Question
Does EK2107 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Mouse Plasma Kallikrein/KLKB1 ELISA Kit PicoKine® (EK2107) doesn't contain native human biological materials. doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Q: is it okay to use heparin plasma as samples in Mouse Plasma Kallikrein Picokine® ELISA Kit (Catalog # EK2107)?
D. White
Verified customer
Asked: 2020-04-04
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Plasma Kallikrein causing degradation of its protein structure. Plasma Kallikrein may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Plasma Kallikrein ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-04-04
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
N. Chakrabarti
Verified customer
Asked: 2016-08-30
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-08-30
