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Product Info Summary
| SKU: | M04533 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Rat |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Pirh2 RCHY1 Monoclonal Antibody
SKU/Catalog Number
M04533
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Pirh2 RCHY1 Monoclonal Antibody catalog # M04533. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Pirh2 RCHY1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04533)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
1H10
Isotype
IgG
Immunogen
Purified recombinant fragment of human Pirh2 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M04533 is reactive to RCHY1 in Human, Rat
Reconstitution
Calculated molecular weight
30110 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04533 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
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Figure 4. Flow Cytometry validation of RCHY1 using Anti-Pirh2 RCHY1 Monoclonal Antibody (M04533).
Flow cytometric (FCM) analysis of PC-12 cells using Pirh2 Monoclonal Antibody (blue) and negative control (red).
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Figure 3. Immunofluorescent staining data of RCHY1 using Anti-Pirh2 RCHY1 Monoclonal Antibody (M04533).
Immunofluorescence (IF) analysis of HeLa cells using Pirh2 Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Figure 2. Immunohistochemistry validation of RCHY1 using Anti-Pirh2 RCHY1 Monoclonal Antibody (M04533).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Tonsil tissues with AEC staining using Pirh2 Monoclonal Antibody.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-Pirh2 RCHY1 Monoclonal Antibody M04533
Western Blot (WB) analysis using Pirh2 Monoclonal Antibody against HeLa (1)Protein Target Info & Infographic
Gene/Protein Information For RCHY1 (Source: Uniprot.org, NCBI)
Gene Name
RCHY1
Full Name
RING finger and CHY zinc finger domain-containing protein 1
Weight
30110 MW
Alternative Names
RING finger and CHY zinc finger domain-containing protein 1;2.3.2.27;Androgen receptor N-terminal-interacting protein;CH-rich-interacting match with PLAG1;E3 ubiquitin-protein ligase Pirh2;RING finger protein 199;RING-type E3 ubiquitin transferase RCHY1;Zinc finger protein 363;p53-induced RING-H2 protein;hPirh2;RCHY1;ARNIP, CHIMP, PIRH2, RNF199, ZNF363; RCHY1 ARNIP, CHIMP, PIRH2, PRO1996, RNF199, ZCHY, ZNF363 ring finger and CHY zinc finger domain containing 1 RING finger and CHY zinc finger domain-containing protein 1|CH-rich interacting match with PLAG1|E3 ubiquitin-protein ligase Pirh2|RING finger protein 199|RING-type E3 ubiquitin transferase RCHY1|androgen-receptor N-terminal-interacting protein|p53-induced protein with a RING-H2 domain|ring finger and CHY zinc finger domain containing 1, E3 ubiquitin protein ligase|zinc finger protein 363|zinc finger, CHY-type
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RCHY1, check out the RCHY1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RCHY1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Pirh2 RCHY1 Monoclonal Antibody (M04533)
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