Anti-Pirh2 RCHY1 Monoclonal Antibody

Product Name

Anti-Pirh2 RCHY1 Monoclonal Antibody

View all Pirh2 Antibodies

SKU/Catalog Number

M04533

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Pirh2 RCHY1 Monoclonal Antibody catalog # M04533. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Pirh2 RCHY1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04533)

Host

Mouse

Contents

Ascitic fluid containing 0.03% sodium azide.

Clonality

Monoclonal

Clone Number

1H10

Isotype

IgG

Immunogen

Purified recombinant fragment of human Pirh2 expressed in E. Coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M04533 is reactive to RCHY1 in Human, Rat

Reconstitution

Observed Molecular Weight

Calculated molecular weight

30110 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M04533 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000

Positive Control

Validation Images & Assay Conditions

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Figure 4. Flow Cytometry validation of RCHY1 using Anti-Pirh2 RCHY1 Monoclonal Antibody (M04533).

Flow cytometric (FCM) analysis of PC-12 cells using Pirh2 Monoclonal Antibody (blue) and negative control (red).

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Figure 3. Immunofluorescent staining data of RCHY1 using Anti-Pirh2 RCHY1 Monoclonal Antibody (M04533).

Immunofluorescence (IF) analysis of HeLa cells using Pirh2 Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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Figure 2. Immunohistochemistry validation of RCHY1 using Anti-Pirh2 RCHY1 Monoclonal Antibody (M04533).

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Tonsil tissues with AEC staining using Pirh2 Monoclonal Antibody.
For more protocol information of IHC

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Figure 1. Western blotting validation for Anti-Pirh2 RCHY1 Monoclonal Antibody M04533

Western Blot (WB) analysis using Pirh2 Monoclonal Antibody against HeLa (1)

Gene/Protein Information For RCHY1 (Source: Uniprot.org, NCBI)

Alternative Names

Androgen receptor N-terminal-interacting protein; androgen-receptor N-terminal-interacting protein; ARNIPRING finger protein 199; CHIMPRNF199DKFZp586C1620; CH-rich interacting match with PLAG1; CH-rich-interacting match with PLAG1; E3 ubiquitin-protein ligase Pirh2; EC 6.3.2.-; hARNIP; hPirh2; PIRH2; PRO1996; ring finger and CHY zinc finger domain containing 1; RING finger and CHY zinc finger domain-containing protein 1; Zinc finger protein 363p53-induced RING-H2 protein; ZNF363 RCHY1 ARNIP, CHIMP, PIRH2, PRO1996, RNF199, ZCHY, ZNF363 ring finger and CHY zinc finger domain containing 1 RING finger and CHY zinc finger domain-containing protein 1|CH-rich interacting match with PLAG1|E3 ubiquitin-protein ligase Pirh2|RING finger protein 199|RING-type E3 ubiquitin transferase RCHY1|androgen-receptor N-terminal-interacting protein|p53-induced protein with a RING-H2 domain|ring finger and CHY zinc finger domain containing 1, E3 ubiquitin protein ligase|zinc finger protein 363|zinc finger, CHY-type

For more info on RCHY1, check out the RCHY1 Infographic

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In this infographic, you will see the following information for RCHY1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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