Anti-PINX1 Antibody Picoband™

Product Name

Anti-PINX1 Antibody Picoband™

View all PINX1 Antibodies

SKU/Catalog Number

PB9498

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PINX1 Antibody Picoband™ catalog # PB9498. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PINX1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9498)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human PINX1, different from the related mouse and rat sequences by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9498 is reactive to PINX1 in Human

Applications

PB9498 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

37 kDa

Calculated molecular weight

37035 MW

Background of PINX1

PINX1, also known as PIN2 interacting protein 1, is a telomerase inhibitor and a possible tumor suppressor. It is mapped to 8p23. Over-expression of PINX1 results in decreased telomerase activity, telomere shortening, and induction of crisis. Reduction of PINX1 leads to an increase in telomerase activity and elongation of telomeres. PINX1 differs from other proteins that regulate telomere length in that it acts on telomerase while other proteins adjust telomere length without affecting telomerase activity. The PINX1 budding yeast orthologue Gnop1 inhibits telomerase by isolating the uncomplexed TERT protein so that it cannot associate with the telomerase template RNA, which prevents telomerase from being assembled. However, in humans, PINX1 impedes already assembled telomerase.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PINX1 using anti-PINX1 antibody (PB9498).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Human Placenta Tissue Lysate at 50ug,
Lane 2: HELA Whole Cell Lysate at 40ug,
Lane 3: HUT Whole Cell Lysate at 40ug,
Lane 4: JURKAT Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PINX1 antigen affinity purified polyclonal antibody (Catalog # PB9498) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PINX1 at approximately 37 kDa. The expected band size for PINX1 is at 37 kDa.

Gene/Protein Information For PINX1 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ20565; hepatocellular carcinoma-related putative tumor suppressor; Liver-related putative tumor suppressor; LPTLPinX1; LPTS67-11-3 protein; MGC8850; PIN2 interacting protein 1; PIN2/TERF1 interacting, telomerase inhibitor 1; PIN2/TERF1-interacting telomerase inhibitor 1; PIN2-interacting protein 1; Pin2-interacting protein X1; Protein 67-11-3; TRF1-interacting protein 1 PINX1 Gno1, LPTL, LPTS, Pxr1 PIN2 (TERF1) interacting telomerase inhibitor 1 PIN2/TERF1-interacting telomerase inhibitor 1|67-11-3 protein|PIN2-interacting protein 1|TRF1-interacting protein 1|hepatocellular carcinoma-related putative tumor suppressor|liver-related putative tumor suppressor|pin2-interacting protein X1|protein 67-11-3

For more info on PINX1, check out the PINX1 Infographic

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In this infographic, you will see the following information for PINX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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