PINX1 Antibodies

PIN2/TERF1-interacting telomerase inhibitor 1 (PINX1)

Microtubule-binding protein essential for faithful chromosome segregation. Mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres.

Inhibits telomerase activity. May inhibit cell proliferation and act as tumor suppressor.

Gene Information

Human
Gene Name:	PINX1
Uniprot:	Q96BK5
Entrez:	54984

Family

Belongs to:
PINX1 family

Alternative Names

FLJ20565; hepatocellular carcinoma-related putative tumor suppressor; Liver-related putative tumor suppressor; LPTLPinX1; LPTS67-11-3 protein; MGC8850; PIN2 interacting protein 1; PIN2/TERF1 interacting, telomerase inhibitor 1; PIN2/TERF1-interacting telomerase inhibitor 1; PIN2-interacting protein 1; Pin2-interacting protein X1; Protein 67-11-3; TRF1-interacting protein 1

Molecular Weight

Mass (kDA):
37.035 kDA

Genomic Context

Human
Location:	8p23.1
Sequence:	8; NC_000008.11 (10764961..10839899, complement)

Tissue Specificity

Ubiquitous; expressed at low levels. Not detectable in a number of hepatocarcinoma cell lines.

Subcellular Localizations

Nucleus. Nucleus, nucleolus. Chromosome, telomere. Chromosome, centromere, kinetochore. Localizes in nucleoli, at telomere speckles and to the outer plate of kinetochores. Localization to the kinetochore is mediated by its central region and depends on NDC80 and CENPE.

Diseases

• Malignant Neoplasms
• Neoplasms
• Carcinoma
• Telomere Shortening
• Liver Carcinoma
• Stomach Neoplasms
• Cell Transformation, Neoplastic
• Liver Neoplasms
• Malignant Neoplasm Of Stomach
• Mammary Neoplasms
• Leukemia
• Neoplasm Metastasis

• Malignant Neoplasm Of Breast
• Instabilities, Chromosomal
• Metastatic Malignant Neoplasm To The Lymph Node
• Neoplasms, Experimental
• Hepatitis B
• Immunologic Deficiency Syndromes

Interacting Proteins

• TERF1

Pathways

• Localization
• Telomere Maintenance
• Mitosis
• Cell Growth
• Cell Proliferation
• Senescence
• Cell Cycle
• S Phase
• Rna Interference
• Cellular Senescence
• Aging
• Chromosome Segregation
• Anaphase

• Interphase
• Oncogenesis
• Regulation Of Telomerase Activity
• Methylation
• Cell Death
• Rna Processing
• Cell Migration

PTMs

• Phosphorylation
• Acetylation

• Methylation
• Ubiquitination

• Reduction

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PINX1

Boster Bio - Best Uses Of The PINX1 Marker

Boster has your back if you're looking to find the best primary antibody against PINX1. Their antibodies are high-affinity, have been highly cited for over 25 years, and are backed by solid validation on immunohistochemistry, Western blotting, and ELISA. If you're looking for high-affinity antibodies against PINX1, read on for some helpful tips and questions to ask Steven Boster.

Primary antibodies with high affinity

PinX1 plays a crucial role in tumor invasions and metastasis, in addition to its role as a regulator of cancer progression. In breast cancer, increased expression of MMP-2 and MMP-9 is associated with poorer survival. PinX1 stops breast cancer cells from migrating and invading by inhibiting MMP-9. The tissue inhibitors for metalloproteinases, (TIMPs), also inhibit the protein.

After affinity-purification, the anti-mPARP-2 antibody was used to detect hTRF2. The antibody was then diluted to 3%. Similar to the anti-hTRF2 antibodies, the PINX1 marker was used to prepare the antibody and it was used at 1:20 dilution. Incubation time for both antibodies was 90 minutes. The diluted antibodies were then used to perform immunoblot analyses of PINX1-positive and PINX1-negative follicle cells.

Two pathologists obtained the results of the study. Two pathologists rated the intensity and color of the PinX1 staining. Positive cells mainly exhibited staining of the PINX1 marker in the nucleus. Positive cells also showed some staining within the cytoplasm. The intensity of PinX1 stains was rated according to the number of cells showing positive staining. The percentage of positive cells was then converted to an immunoreactive score. This score was calculated based on multiplying the intensity staining by positive cells.

Low PinX1 levels are associated with poor prognosis and breast cancer. Low PinX1 expression may be a prognostic indicator for breast cancer. It is associated with increased tumor cells metastasis. Therefore, high-affinity antibodies using the PINX1 marker may prove to be a valuable asset for breast cancer treatment. This marker will allow researchers to identify new targets for the drug.

The importance of SHM in antibody maturation has been revealed by molecular analysis of several CSR-deficient mice. This involves genetic modification to B cells via stochastic changes in the V area of Ig. This mutation can result in multiple types of antibodies with the same antigen affinity. The PINX1 mutant also enhanced the migration abilities of the PinX1KDMDA-MB-231, BT-549 and BT-549 lines. SHM may regulate immune responses by regulating tumor cell invasion and migration.

The PINX1 protein is an important regulator for telomere length. The protein inhibits telomerase, and nucleophosmin attenuates this inhibition. This interaction could be crucial for cancer treatment as it is thought to be a prognostic indicator for non-small-cell lung cancer. PinX1 is a gene that regulates the activity of telomerase, hTERT, and other functions.

PinX1 colocalizes to NPM in S-phase and plays an essential part in telomere homeostasis. It was studied by performing endogenous immunofluorescence using HeLa cells synchronized to the G1/S boundary. Treatment with Hydroxyurea released a block 16 to 18 h after treatment. The cells were stained in antibodies.