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Product Info Summary
| SKU: | PB9776 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PIGR Antibody Picoband®
SKU/Catalog Number
PB9776
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PIGR Antibody Picoband® catalog # PB9776. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PIGR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9776)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human PIGR, different from the related rat sequence by eighteen amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9776 is reactive to PIGR in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
83 kDa
Calculated molecular weight
83284 MW
Background of PIGR
Polymeric immunoglobulin receptor is a protein that in humans is encoded by the PIGR gene. It is a Fc receptor which facilitates the secretion of the soluble polymeric isoforms of immunoglobulin A and immunoglobulin M. This gene is mapped to 1q31-q41. The encoded poly-Ig receptor binds polymeric immunoglobulin molecules at the basolateral surface of epithelial cells; the complex is then transported across the cell to be secreted at the apical surface. A significant association was found between immunoglobulin A nephropathy and several SNPs in this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9776 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell, SKOV Whole Cell, MCF-7 Whole Cell, SW620 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PIGR using anti-PIGR antibody (PB9776).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: HELA Whole Cell Lysate,
Lane 2: SKOV Whole Cell Lysate,
Lane 3: MCF-7 Whole Cell Lysate,
Lane 4: SW620 Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIGR antigen affinity purified polyclonal antibody (Catalog # PB9776) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIGR at approximately 83 kDa. The expected band size for PIGR is at 83 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PIGR (Source: Uniprot.org, NCBI)
Gene Name
PIGR
Full Name
Polymeric immunoglobulin receptor
Weight
83284 MW
Alternative Names
Polymeric immunoglobulin receptor;PIgR;Poly-Ig receptor;Hepatocellular carcinoma-associated protein TB6;Secretory component;PIGR; PIGR polymeric immunoglobulin receptor polymeric immunoglobulin receptor|hepatocellular carcinoma associated protein TB6|poly-Ig receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PIGR, check out the PIGR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PIGR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PIGR Antibody Picoband® (PB9776)
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No publications found for PB9776
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-PIGR Antibody Picoband®
Question
Is a blocking peptide available for product anti-PIGR antibody (PB9776)?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
We do provide the blocking peptide for product anti-PIGR antibody (PB9776). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-07
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tear using anti-PIGR antibody PB9776. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-03
Question
I see that the anti-PIGR antibody PB9776 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-14
Question
We want to test anti-PIGR antibody PB9776 on human tear for research purposes, then I may be interested in using anti-PIGR antibody PB9776 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
The products we sell, including anti-PIGR antibody PB9776, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-12-18
Question
Is there a BSA free version of anti-PIGR antibody PB9776 available?
Verified Customer
Verified customer
Asked: 2017-12-14
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PIGR antibody PB9776 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-14
Question
We are currently using anti-PIGR antibody PB9776 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-25
Answer
The anti-PIGR antibody (PB9776) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-25
