Click image to see more details
Product Info Summary
| SKU: | EKC1458 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
Customers Who Bought This Also Bought
Product info
Product Name
PIAS3 Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC1458
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The PIAS3 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PIAS3 protein expression profile in cells. The kit can be used for measuring the relative amounts of PIAS3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PIAS3.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
PIAS3 Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1458)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1458 is reactive to PIAS3 in Human, Mouse, Rat samples
Application Guarantee
EKC1458 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-PIAS3 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
Click image to see more details
Boster Kit Box
Click image to see more details
Western blot analysis of extracts from 293 cells
Protein Target Info & Infographic
Gene/Protein Information For PIAS3 (Source: Uniprot.Org, NCBI)
Gene Name
PIAS3
Full Name
E3 SUMO-protein ligase PIAS3
Weight
68017 MW
Superfamily
PIAS family
Alternative Names
E3 SUMO-protein ligase PIAS3;2.3.2.-;E3 SUMO-protein transferase PIAS3 ;Protein inhibitor of activated STAT protein 3;PIAS3; PIAS3 ZMIZ5 protein inhibitor of activated STAT 3 E3 SUMO-protein ligase PIAS3|E3 SUMO-protein transferase PIAS3|protein inhibitor of activated STAT protein 3|zinc finger, MIZ-type containing 5
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PIAS3, check out the PIAS3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PIAS3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PIAS3 Colorimetric Cell-Based ELISA Kit (EKC1458)
Hello CJ!
No publications found for EKC1458
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used PIAS3 Colorimetric Cell-Based ELISA Kit?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For PIAS3 Colorimetric Cell-Based ELISA Kit
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
