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- Table of Contents
Facts about E3 SUMO-protein ligase PIAS3.
Involved in regulating STAT3 signaling through inhibiting STAT3 DNA-binding and controlling cell growth. Enhances the sumoylation of MTA1 and might participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184).
Human | |
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Gene Name: | PIAS3 |
Uniprot: | Q9Y6X2 |
Entrez: | 10401 |
Belongs to: |
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PIAS family |
MIZ-type containing 5; PIAS3; Protein inhibitor of activated STAT protein 3; protein inhibitor of activated STAT, 3; ZMIZ5
Mass (kDA):
68.017 kDA
Human | |
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Location: | 1q21.1 |
Sequence: | 1; NC_000001.11 (145848522..145859081, complement) |
Widely expressed.
Cytoplasm. Nucleus. Nucleus speckle. Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules.
The PIAS3 Cell-Based ELISA Kit is an excellent tool for screening the expression profile of PIAS3 in cells. This kit measures the relative levels of PIAS3 in cultured cells and is suitable for screening the effects of treatments or activators. It is not available for individual purchase and is therefore only applicable to Boster products. However, PIAS3 is a widely recognized biomarker, and as such, its detection is essential for the study of its biological significance.
The PIAS3 Cell-Based ELSA Kit from Boster Bio is a high-throughput, convenient tool for analyzing the expression profile of PIAS3 in cells. Whether you want to screen the effect of a drug or an activator on PIAS3, this kit can help. But, it is important to note that this kit is not available individually. The kit only applies to Boster products.
This enzyme-linked immunosorbent assay (ELISA) is a technique for detecting protein concentrations in liquid samples. It has three types of data output: the concentration of protein in the sample, the amount of antigen, and the specific antibody that recognizes the antigen. The PIAS3 Cell-Based ELISA Kit from Boster Bio provides you with all three types of data output.
PIAS3 is a fluorescent tag that has been used to identify high-affinity primary antibodies. It is a unique marker that binds to a particular epitope on a tissue section. The target of the antibody must be accessible to the specific antibody to recognize it. However, it is not entirely clear how high-affinity primary antibodies can be produced. The PIAS3 marker may help researchers identify high-affinity primary antibodies that are capable of detecting epitopes on fixed tissue sections.
The PIAS3 marker allows researchers to identify high-affinity primary antibodies that bind to NP-binding antigens. PIAS3-markers have also been found to be highly specific to DNA. These antibodies were developed from human peripheral blood monoclonal antigens. These antibodies recognize the NP-binding antigens and inhibit the production of IgG and IgM antibodies.
The PIAS3 protein is 97% identical to human PIAS3 and is also highly similar to mouse and canine proteins. It contains aa 1-628 and aa 10-628. The human PIAS3 is 97% identical to mouse and canine PIAS3, and the two are structurally equivalent. It is highly conserved and stable under various conditions, including heat, which makes it a very useful tool for immunohistochemistry.
This marker is essential for producing a high-affinity primary antibody that recognizes PIAS3 antigens. The antibody secreting cells lack the typical markers of B1-B cells. The absence of IgD-class BCRs suggests that the cells secreting antibodies to PIAS3-reactive antigens are prone to rapid primary immune responses. This research also provides the first proof-of-concept that PIAS3-based antibodies can be highly specific and useful.
In this study, mice with IgD deficiency were injected with InsA-KLH. The serum of these mice was analyzed on day 0, day 21, and day 42 for high-affinity IgM. The data presented are means + SD for three independent experiments. So, PIAS3-deficient mice are more likely to produce antibodies with high affinity to insulin.
The IgD BCR regulates the secretion of antibodies. It senses antigen-valence, which in turn controls the duration of the antibody response. A monovalent antigen inhibits the production of IgG, while a polyvalent antigen evokes an efficient IgM memory response from activated mature B cells. This regulation is critical for the maturation of antibody responses and tolerance to self-structures.
The PIAS3 protein is a nuclear E3-type SUMO ligase. It has the ability to inhibit the gene activation activities of STAT3, MITF, and progesterone receptor. It is 65-75 kDa and contains the SAP domain, STAT3 binding site, and PINIT motif. The PIAS3 protein binds to a wide range of proteins, including DNA, RNA, and protein. The human PIAS3 protein is 628 amino acids long and contains two domains, an SAP domain and an STAT3-binding site. One domain is a zinc finger motif called a PINIT motif.
PMID: 10319586 by Ueki N., et al. Isolation and chromosomal assignment of a human gene encoding protein inhibitor of activated STAT3 (PIAS3).
PMID: 9388184 by Chung C.D., et al. Specific inhibition of Stat3 signal transduction by PIAS3.