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Product Info Summary
| SKU: | A04130-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-PIAS2 Antibody Picoband®
SKU/Catalog Number
A04130-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PIAS2 Antibody Picoband® catalog # A04130-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PIAS2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04130-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PIAS2 recombinant protein (Position: E93-K311).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04130-3 is reactive to PIAS2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
68 kDa
Calculated molecular weight
211344 MW
Background of PIAS2
E3 SUMO-protein ligase PIAS2 is an enzyme that in humans is encoded by the PIAS2 gene. It is mapped to 18q21.1. This gene encodes a member of the protein inhibitor of activated STAT family, which function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Isoforms of the encoded protein enhance the sumoylation of specific target proteins including the p53 tumor suppressor protein, c-Jun, and the androgen receptor. A pseudogene of this gene is located on the short arm of chromosome 4. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04130-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human K562 whole cell, human Hela whole cell, human HepG2 whole cell, human HEK293 whole cell, human HL-60 whole cell, human Raji whole cell, human A431 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse testis tissue, mouse Neuro-2a whole cell, mouse RAW2647 whole cell
FCM: U937 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PIAS2 using anti-PIAS2 antibody (A04130-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human HEK293 whole cell lysates,
Lane 5: human HL-60 whole cell lysates,
Lane 6: human Raji whole cell lysates,
Lane 7: human A431 whole cell lysates,
Lane 8: rat brain tissue lysates,
Lane 9: rat C6 whole cell lysates,
Lane 10: mouse brain tissue lysates,
Lane 11: mouse testis tissue lysates,
Lane 12: mouse Neuro-2a whole cell lysates,
Lane 13: mouse RAW264.7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIAS2 antigen affinity purified polyclonal antibody (Catalog # A04130-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIAS2 at approximately 68KD. The expected band size for PIAS2 is at 68KD.
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Figure 2. Flow Cytometry analysis of U937 cells using anti-PIAS2 antibody (A04130-3).
Overlay histogram showing U937 cells stained with A04130-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PIAS2 Antibody (A04130-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For PIAS2 (Source: Uniprot.org, NCBI)
Gene Name
PIAS2
Full Name
E3 SUMO-protein ligase PIAS2
Weight
211344 MW
Superfamily
PIAS family
Alternative Names
E3 SUMO-protein ligase PIAS2; Androgen receptor-interacting protein 3; ARIP3; DAB2-interacting protein; DIP; E3 SUMO-protein transferase PIAS2; Msx-interacting zinc finger protein; Miz1; PIAS-NY protein; Protein inhibitor of activated STAT x; Protein inhibitor of activated STAT2; PIAS2; PIASX PIAS2 ARIP3, DIP, MIZ1, PIASX, SIZ2, ZMIZ4 protein inhibitor of activated STAT 2 E3 SUMO-protein ligase PIAS2|DAB2-interacting protein|E3 SUMO-protein transferase PIAS2|androgen receptor-interacting protein 3|msx-interacting zinc finger protein|protein inhibitor of activated STAT X|zinc finger, MIZ-type containing 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PIAS2, check out the PIAS2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PIAS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PIAS2 Antibody Picoband® (A04130-3)
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