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Product Info Summary
| SKU: | M02221 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PHOX2B Monoclonal Antibody
SKU/Catalog Number
M02221
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PHOX2B Monoclonal Antibody catalog # M02221. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PHOX2B Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02221)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEFG-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PHOX2B Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02221 is reactive to PHOX2B in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
90 kDa
Calculated molecular weight
57235 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02221 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:500
ICC/IF 1:50-1:200
Positive Control
WB: Neuro-2a cell
Validation Images & Assay Conditions
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Western blot analysis of PHOX2B expression in Neuro-2a cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PHOX2B (Source: Uniprot.org, NCBI)
Gene Name
PHOX2B
Full Name
Paired mesoderm homeobox protein 2B
Weight
57235 MW
Superfamily
paired homeobox family
Alternative Names
CD276 antigen;4Ig-B7-H3;B7 homolog 3;B7-H3;Costimulatory molecule;CD276;CD276;B7H3;PSEC0249, UNQ309/PRO352; PHOX2B CCHS, NBLST2, NBPhox, PMX2B paired like homeobox 2B paired mesoderm homeobox protein 2B|PHOX2B homeodomain protein|neuroblastoma Phox|neuroblastoma paired-type homeobox protein|paired like homeobox2B|paired mesoderm homeobox 2b
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PHOX2B, check out the PHOX2B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PHOX2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PHOX2B Monoclonal Antibody (M02221)
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No publications found for M02221
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Customer Q&As
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2 Customer Q&As for Anti-PHOX2B Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M02221?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of PHOX2B expression in Neuro-2a cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
We are currently using anti-PHOX2B Monoclonal antibody M02221 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-22
Answer
The anti-PHOX2B Monoclonal antibody (M02221) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-22
