Anti-PHOX2B Antibody

Product Name

Anti-PHOX2B Antibody

View all PHOX2B Antibodies

SKU/Catalog Number

A02221

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-PHOX2B Antibody (Catalog # A02221). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

PHOX2B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-PHOX2B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02221)

Host

Rabbit

Contents

PHOX2B Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

PHOX2B antibody was raised against a 15 amino acid synthetic peptide near the center of human PHOX2B. The immunogen is located within amino acids 130 - 180 of PHOX2B.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

PHOX2B antibody is predicted to not cross-react with other paired homeobox family members.

Reactive Species

A02221 is reactive to PHOX2B in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

31621 MW

Background of PHOX2B

PHOX2A and PHOX2B (Paired mesoderm homeobox protein) are closely related, paired-homeodomain transcription factors that function as determinants of the noradrenergic phenotype during embryogenesis. PHOX2 proteins are crucial for the regulation of endogenous hydroxylases in neural crest cells and promote sympathetic neuron generation. Human PHOX2B contains one DNA binding homeobox domain and is required for the differentiation of all central and nonperipheral noradrenergic centers in the brain. In contrast, PHOX2A controls only the differentiation of the main noradrenergic center of the brain. Regulation of PHOX2 may have therapeutic utility in aging or disorders involving degeneration of noradrenergic neurons.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A02221 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PHOX2B antibody can be used for detection of PHOX2B by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For PHOX2B (Source: Uniprot.org, NCBI)

Alternative Names

PMX2B, NBLST2, NBPhox, PMX2B, Paired mesoderm homeobox protein 2B, Neuroblastoma Phox PHOX2B CCHS, NBLST2, NBPhox, PMX2B paired like homeobox 2B paired mesoderm homeobox protein 2B|PHOX2B homeodomain protein|neuroblastoma Phox|neuroblastoma paired-type homeobox protein|paired like homeobox2B|paired mesoderm homeobox 2b

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis