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Product Info Summary
| SKU: | A01207Y821 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC |
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Product info
Product Name
Anti-Phospho-Vinculin (Y821) VCL Antibody
SKU/Catalog Number
A01207Y821
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Vinculin (Y821) VCL Antibody catalog # A01207Y821. Tested in ELISA, IF, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Vinculin (Y821) VCL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01207Y821)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Vinculin around the phosphorylation site of Tyr821. AA range:786-835
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01207Y821 is reactive to VCL in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
123799 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01207Y821 is guaranteed for ELISA, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Vinculin (Phospho-Tyr821) antibody
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Immunofluorescence analysis of HepG2 cells, using Vinculin (Phospho-Tyr821) antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Vinculin (Phospho-Tyr821) antibody. The picture on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For VCL (Source: Uniprot.org, NCBI)
Gene Name
VCL
Full Name
Vinculin
Weight
123799 MW
Superfamily
vinculin/alpha-catenin family
Alternative Names
Vinculin;Metavinculin;MV;VCL; VCL CMD1W, CMH15, HEL114, MV, MVCL vinculin vinculin|epididymis luminal protein 114|epididymis secretory sperm binding protein|meta-vinculin|metavinculin|vinculin isoform meta-VCL
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VCL, check out the VCL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VCL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Vinculin (Y821) VCL Antibody (A01207Y821)
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