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Product Info Summary
| SKU: | P04119 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-TOB1 (S164) Antibody
SKU/Catalog Number
P04119
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Phospho-TOB1 (S164) Antibody catalog # P04119. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-TOB1 (S164) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P04119)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human TOB1 around the phosphorylation site of Ser164. AA range:130-179
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P04119 is reactive to TOB1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
38155 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P04119 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot analysis of JK cells using Phospho-TOB1 (S164) polyclonal antibody
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Immunohistochemistry analysis of paraffin-embedded human brain, using TOB1 (Phospho-Ser164) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from HT29 cells treated with serum 20% 15', using TOB1 (Phospho-Ser164) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For TOB1 (Source: Uniprot.org, NCBI)
Gene Name
TOB1
Full Name
Protein Tob1
Weight
38155 MW
Superfamily
BTG family
Alternative Names
Protein Tob1;Transducer of erbB-2 1;TOB1;TOB, TROB1; TOB1 APRO5, APRO6, PIG49, TOB, TROB, TROB1 transducer of ERBB2, 1 protein Tob1|proliferation-inducing gene 49|transducer of erbB-2 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TOB1, check out the TOB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TOB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-TOB1 (S164) Antibody (P04119)
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No publications found for P04119
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Customer Q&As
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6 Customer Q&As for Anti-Phospho-TOB1 (S164) Antibody
Question
Do you have a BSA free version of anti-Phospho-TOB1 (S164) antibody P04119 available?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-TOB1 (S164) antibody P04119 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-30
Question
My question regarding product P04119, anti-Phospho-TOB1 (S164) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P04119 anti-Phospho-TOB1 (S164) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-19
Question
We are currently using anti-Phospho-TOB1 (S164) antibody P04119 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
The anti-Phospho-TOB1 (S164) antibody (P04119) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-25
Question
Does anti-Phospho-TOB1 (S164) antibody P04119 work on bovine WB with cervix carcinoma?
J. Patel
Verified customer
Asked: 2019-06-04
Answer
Our lab technicians have not validated anti-Phospho-TOB1 (S164) antibody P04119 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Phospho-TOB1 (S164) antibody P04119 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-04
Question
I see that the anti-Phospho-TOB1 (S164) antibody P04119 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-09-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-09-21
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Phospho-TOB1 (S164) antibody P04119. Let me know if you need anything else.
G. Collins
Verified customer
Asked: 2016-09-22
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-09-22
