Product Info Summary
SKU: | A00097S235 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Phospho-Tau (S235) MAPT Antibody
SKU/Catalog Number
A00097S235
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Tau (S235) MAPT Antibody catalog # A00097S235. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Tau (S235) MAPT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00097S235)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Tau around the phosphorylation site of S235.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00097S235 is reactive to MAPT in Human, Mouse, Rat
Applications
A00097S235 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
78928 MW
Background of Tau
Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region.
Kamps M.P., Cell 60:547-555(1990).
Nourse J., Cell 60:535-545(1990).
Grimwood J., Nature 428:529-535(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:20000
Validation Images & Assay Conditions
![a00097s235 anti phospho tau s235 mapt antibody wb testing 1 a00097s235 anti phospho tau s235 mapt antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a00097s235-anti-phospho-tau-s235-mapt-antibody-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blotting validation for Anti-Phospho-Tau (S235) MAPT Antibody A00097S235
Western Blot (WB) analysis of specific cells using Phospho-Tau (S235) polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For MAPT (Source: Uniprot.org, NCBI)
Gene Name
MAPT
Full Name
Microtubule-associated protein tau
Weight
78928 MW
Alternative Names
DDPAC; FLJ31424; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; MAPT; MGC138549; microtubule-associated protein tau; MSTD; MSTDMAPTL; MTBT1; MTBT1Neurofibrillary tangle protein; MTBT2; Neurofibrillary tangle protein; PHF-tau; PPND; Tau; TAUPaired helical filament-tau MAPT DDPAC, FTDP-17L, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, tau-40, MAPT microtubule associated protein tau microtubule-associated protein tau|G protein beta1/gamma2 subunit-interacting factor 1|PHF-tau|neurofibrillary tangle protein|paired helical filament-tau|protein phosphatase 1, regulatory subunit 103
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAPT, check out the MAPT Infographic
![MAPT infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAPT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Tau (S235) MAPT Antibody (A00097S235)
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No publications found for A00097S235
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