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Product Info Summary
| SKU: | P01458 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Phospho-TAK1 (S439) Rabbit Monoclonal Antibody
SKU/Catalog Number
P01458
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Phospho-TAK1 (S439) Rabbit Monoclonal Antibody catalog # P01458. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-TAK1 (S439) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01458)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
28M09
Isotype
IgG
Immunogen
A synthesized peptide derived from human Phospho-TAK1 (S439)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P01458 is reactive to MAP3K7 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
41 kDa
Calculated molecular weight
47 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01458 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human HepG2 whole cell, human A549 whole cell, rat lung tissue, rat brain tissue, mouse lung tissue, mouse brain tissue lysates,
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Phospho-TAK1 using anti-Phospho-TAK1 antibody (P01458).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse brain tissue lysates,
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-TAK1 antigen affinity purified monoclonal antibody (Catalog # P01458) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Phospho-TAK1 at approximately 41 kDa. The expected band size for Phospho-TAK1 is at 47 kDa.
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Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution)
Protein Target Info & Infographic
Gene/Protein Information For MAP3K7 (Source: Uniprot.org, NCBI)
Gene Name
MAP3K7
Full Name
Mitogen-activated protein kinase kinase kinase 7
Weight
47 kDa
Superfamily
protein kinase superfamily
Alternative Names
Tyrosine-protein kinase Lyn;2.7.10.2;Lck/Yes-related novel protein tyrosine kinase;V-yes-1 Yamaguchi sarcoma viral related oncogene homolog;p53Lyn;p56Lyn;LYN;JTK8; MAP3K7 CSCF, FMD2, MEKK7, TAK1, TGF1a mitogen-activated protein kinase kinase kinase 7 mitogen-activated protein kinase kinase kinase 7|TGF-beta activated kinase 1|transforming growth factor-beta-activated kinase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP3K7, check out the MAP3K7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP3K7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-TAK1 (S439) Rabbit Monoclonal Antibody (P01458)
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