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Product Info Summary
| SKU: | P02314-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ICC, WB |
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Product info
Product Name
Anti-Phospho-Synaptotagmin (Ser309) Syt1 Antibody
View all Synaptotagmin 1 Antibodies
SKU/Catalog Number
P02314-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Synaptotagmin (Ser309) Syt1 Antibody (Catalog # P02314-1). Tested in WB, ICC applications. This antibody reacts with Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-Synaptotagmin (Ser309) Syt1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02314-1)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser309 of rat synaptotagmin, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P02314-1 is reactive to SYT1 in Rat
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
47399 MW
Background of Synaptotagmin 1
Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin’s ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P02314-1 is guaranteed for ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
ICC: 1:400
Validation Images & Assay Conditions
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Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Thr202 in red and PSD95 in green.
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Western blot of rat cortical lysate showing specific immunolabeling of the ~62 kDa synaptotagmin phosphorylated at Thr202 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 minutes).
Protein Target Info & Infographic
Gene/Protein Information For SYT1 (Source: Uniprot.org, NCBI)
Gene Name
SYT1
Full Name
Synaptotagmin-1
Weight
47399 MW
Superfamily
synaptotagmin family
Alternative Names
DKFZp781D2042 antibody, FLJ42519 antibody, P65 antibody, SVP65 antibody, synaptotagmin 1 antibody, Synaptotagmin I antibody, SYT antibody, SYT1 antibody, SytI antibody SYT1 BAGOS, P65, SVP65, SYT synaptotagmin 1 synaptotagmin-1|synaptotagmin I|sytI
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYT1, check out the SYT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Synaptotagmin (Ser309) Syt1 Antibody (P02314-1)
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No publications found for P02314-1
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Phospho-Synaptotagmin (Ser309) Syt1 Antibody
Question
We purchased anti-Phospho-Synaptotagmin (Ser309) antibody for WB on hippocampus in a previous experiment. I am using rat, and I plan to use the antibody for IHC next. I was wanting to use examining hippocampus as well as middle temporal gyrus in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
I looked at the website and datasheets of our anti-Phospho-Synaptotagmin (Ser309) antibody and it seems that P02314-1 has been tested on rat in both WB and IHC. Thus P02314-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-30
Question
Will anti-Phospho-Synaptotagmin (Ser309) antibody P02314-1 work for WB with pns?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
According to the expression profile of pns, SYT1 is highly expressed in pns. So, it is likely that anti-Phospho-Synaptotagmin (Ser309) antibody P02314-1 will work for WB with pns.
Boster Scientific Support
Answered: 2020-04-20
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pns using anti-Phospho-Synaptotagmin (Ser309) antibody P02314-1. Let me know if you need anything else.
A. Li
Verified customer
Asked: 2020-03-06
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-06
Question
Can you help my question with product P02314-1, anti-Phospho-Synaptotagmin (Ser309) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P02314-1 anti-Phospho-Synaptotagmin (Ser309) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-13
Question
Is a blocking peptide available for product anti-Phospho-Synaptotagmin (Ser309) antibody (P02314-1)?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
We do provide the blocking peptide for product anti-Phospho-Synaptotagmin (Ser309) antibody (P02314-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-24
Question
Is this P02314-1 anti-Phospho-Synaptotagmin (Ser309) antibody reactive to the isotypes of SYT1?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
The immunogen of P02314-1 anti-Phospho-Synaptotagmin (Ser309) antibody is The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser309 of synaptotagmin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-14
Question
See attached the WB image, lot number and protocol we used for pns using anti-Phospho-Synaptotagmin (Ser309) antibody P02314-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-14
Question
We have observed staining in rat middle temporal gyrus. Any tips? Is anti-Phospho-Synaptotagmin (Ser309) antibody supposed to stain middle temporal gyrus positively?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
From what I have seen in literature middle temporal gyrus does express SYT1. From what I have seen in Uniprot.org, SYT1 is expressed in middle temporal gyrus, amygdala brain, hippocampus, pns, among other tissues. Regarding which tissues have SYT1 expression, here are a few articles citing expression in various tissues:
Amygdala, and Brain, Pubmed ID: 14702039
Hippocampus, Pubmed ID: 17974005
PNS, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-06-12
Question
Our lab were content with the WB result of your anti-Phospho-Synaptotagmin (Ser309) antibody. However we have observed positive staining in pns cytoplasmic vesicle using this antibody. Is that expected? Could you tell me where is SYT1 supposed to be expressed?
O. Johnson
Verified customer
Asked: 2019-02-06
Answer
According to literature, pns does express SYT1. Generally SYT1 expresses in cytoplasmic vesicle, secretory vesicle. Regarding which tissues have SYT1 expression, here are a few articles citing expression in various tissues:
Amygdala, and Brain, Pubmed ID: 14702039
Hippocampus, Pubmed ID: 17974005
PNS, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-02-06
Question
We are currently using anti-Phospho-Synaptotagmin (Ser309) antibody P02314-1 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says rat. Is it true that the antibody can work on primate tissues as well?
C. Zhang
Verified customer
Asked: 2018-11-13
Answer
The anti-Phospho-Synaptotagmin (Ser309) antibody (P02314-1) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-13
Question
I was wanting to use your anti-Phospho-Synaptotagmin (Ser309) antibody for WB for rat pns on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat pns identification?
Verified Customer
Verified customer
Asked: 2018-06-25
Answer
As indicated on the product datasheet, P02314-1 anti-Phospho-Synaptotagmin (Ser309) antibody has been validated for IHC, WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat pns in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-25
Question
Will P02314-1 anti-Phospho-Synaptotagmin (Ser309) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-02-16
Answer
It shows on the product datasheet, P02314-1 anti-Phospho-Synaptotagmin (Ser309) antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-16
Question
I was wanting to use to test anti-Phospho-Synaptotagmin (Ser309) antibody P02314-1 on rat pns for research purposes, then I may be interested in using anti-Phospho-Synaptotagmin (Ser309) antibody P02314-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Bhatt
Verified customer
Asked: 2016-05-16
Answer
The products we sell, including anti-Phospho-Synaptotagmin (Ser309) antibody P02314-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-05-16
Question
Is there a BSA free version of anti-Phospho-Synaptotagmin (Ser309) antibody P02314-1 available?
N. Moore
Verified customer
Asked: 2013-08-08
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-Synaptotagmin (Ser309) antibody P02314-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-08-08
Question
Does anti-Phospho-Synaptotagmin (Ser309) antibody P02314-1 work on primate IHC with hippocampus?
K. Wu
Verified customer
Asked: 2013-08-02
Answer
Our lab technicians have not validated anti-Phospho-Synaptotagmin (Ser309) antibody P02314-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Phospho-Synaptotagmin (Ser309) antibody P02314-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate hippocampus in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-08-02
Question
I see that the anti-Phospho-Synaptotagmin (Ser309) antibody P02314-1 works with WB, what is the protocol used to produce the result images on the product page?
H. Gonzalez
Verified customer
Asked: 2013-01-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-01-17
