Product Info Summary
SKU: | P03794-2 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Phospho-Synapsin I (Ser62,67) Syn1 Antibody
View all Synapsin I Antibodies
SKU/Catalog Number
P03794-2
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Synapsin I (Ser62, 67) Syn1 Antibody (Catalog # P03794-2). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-Synapsin I (Ser62,67) Syn1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03794-2)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser62/67 of rat synapsin, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P03794-2 is reactive to SYN1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
74097 MW
Background of Synapsin I
Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Ser-549 along with Ser-62 and Ser-67 are the sites of Synapsin I that are phosphorylated by MAP kinase (Czernik et al., 1987; Jovanovic et al., 1996).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P03794-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
Click image to see more details
Western blot of rat cortical lysate showing specific labeling of the ~78 kDa synapsin protein phosphorylated at Ser62,67 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).
Protein Target Info & Infographic
Gene/Protein Information For SYN1 (Source: Uniprot.org, NCBI)
Gene Name
SYN1
Full Name
Synapsin-1
Weight
74097 MW
Superfamily
synapsin family
Alternative Names
Brain protein 4.1 antibody, SYN 1 antibody, SYN 1a antibody, SYN 1b antibody, SYN I antibody, SYN1 antibody, SYN1_HUMAN antibody, SYN1a antibody, SYN1b antibody, Synapsin 1 antibody, Synapsin I antibody, Synapsin-1 antibody, Synapsin1 antibody, SynapsinI antibody, SYNI antibody SYN1 EPILX, MRX50a, SYN1b, SYNI, SYN1 synapsin I synapsin-1|brain protein 4.1|synapsin Ib
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYN1, check out the SYN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Synapsin I (Ser62,67) Syn1 Antibody (P03794-2)
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No publications found for P03794-2
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Customer Q&As
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6 Customer Q&As for Anti-Phospho-Synapsin I (Ser62,67) Syn1 Antibody
Question
I am interested in to test anti-Phospho-Synapsin I (Ser62,67) antibody P03794-2 on rat anterior cingulate cortex for research purposes, then I may be interested in using anti-Phospho-Synapsin I (Ser62,67) antibody P03794-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
The products we sell, including anti-Phospho-Synapsin I (Ser62,67) antibody P03794-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-12
Question
We have been able to see staining in rat brain cortex. Are there any suggestions? Is anti-Phospho-Synapsin I (Ser62,67) antibody supposed to stain brain cortex positively?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
Based on literature brain cortex does express SYN1. Based on Uniprot.org, SYN1 is expressed in anterior cingulate cortex, brain, brain cortex, among other tissues. Regarding which tissues have SYN1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2110562, 15772651
Brain cortex, Pubmed ID: 15822905
Boster Scientific Support
Answered: 2020-02-18
Question
Our lab were well pleased with the WB result of your anti-Phospho-Synapsin I (Ser62,67) antibody. However we have observed positive staining in anterior cingulate cortex cell junction using this antibody. Is that expected? Could you tell me where is SYN1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
From what I have seen in literature, anterior cingulate cortex does express SYN1. Generally SYN1 expresses in cell junction, synapse. golgi apparatus. Regarding which tissues have SYN1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2110562, 15772651
Brain cortex, Pubmed ID: 15822905
Boster Scientific Support
Answered: 2019-07-26
Question
I was wanting to use your anti-Phospho-Synapsin I (Ser62,67) antibody for WB for rat anterior cingulate cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat anterior cingulate cortex identification?
Verified Customer
Verified customer
Asked: 2019-03-28
Answer
As indicated on the product datasheet, P03794-2 anti-Phospho-Synapsin I (Ser62,67) antibody has been validated for WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat anterior cingulate cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-28
Question
We are currently using anti-Phospho-Synapsin I (Ser62,67) antibody P03794-2 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says rat. Is it likely that the antibody can work on bovine tissues as well?
S. Zhao
Verified customer
Asked: 2015-01-02
Answer
The anti-Phospho-Synapsin I (Ser62,67) antibody (P03794-2) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-01-02
Question
Is this P03794-2 anti-Phospho-Synapsin I (Ser62,67) antibody reactive to the isotypes of SYN1?
C. Jones
Verified customer
Asked: 2013-07-19
Answer
The immunogen of P03794-2 anti-Phospho-Synapsin I (Ser62,67) antibody is The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser62,67 of synapsin I. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-07-19