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Key=image_label, value=Western blot of rat cortical lysate showing specific labeling of the ~78 kDa synapsin protein phosphorylated at Ser62,67 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorpt
Key=small_image_label, value=Western blot of rat cortical lysate showing specific labeling of the ~78 kDa synapsin protein phosphorylated at Ser62,67 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorpt
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Key=description, value=Boster Bio Anti-Phospho-Synapsin I (Ser62, 67) Syn1 Antibody (Catalog # P03794-2). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Key=short_description, value=Boster Bio Anti-Phospho-Synapsin I (Ser62, 67) Syn1 Antibody (Catalog # P03794-2). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Key=meta_keyword, value=Anti-Phospho-Synapsin I (Ser62,67) Syn1 Antibody
Key=contents, value=10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Key=background, value=Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Ser-549 along with Ser-62 and Ser-67 are the sites of Synapsin I that are phosphorylated by MAP kinase (Czernik et al., 1987; Jovanovic et al., 1996).
Key=gene_name, value=SYN1
Key=synonyms, value=Brain protein 4.1 antibody, SYN 1 antibody, SYN 1a antibody, SYN 1b antibody, SYN I antibody, SYN1 antibody, SYN1_HUMAN antibody, SYN1a antibody, SYN1b antibody, Synapsin 1 antibody, Synapsin I antibody, Synapsin-1 antibody, Synapsin1 antibody, SynapsinI antibody, SYNI antibody
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Key=subcellular_localization, value= Cell junction, synapse. Golgi apparatus.
Key=tissue_specificity, value= Heart and skeletal muscle specific. Expression is commonly increased in the brain of patients with bipolar disorder, schizophrenia, and major depression. .
Key=protein_function, value= Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. Regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxide functions at a presynaptic level.
Key=application_notes, value=Specific for endogenous levels of the ~78 kDa synapsin I doublet phosphorylated at Ser62,67. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Key=competitor_equivalent_skus, value=sc 82014|sc 82013|sc 81813|sc 133900|sc 82014 X|sc 82013 X
Key=immunogen, value=Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser62/67 of rat synapsin, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
Key=purification, value=Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Key=storage, value=Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
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Anti-Phospho-Synapsin I (Ser62,67) Syn1 Antibody

Synapsin I antibody

Boster Bio Anti-Phospho-Synapsin I (Ser62, 67) Syn1 Antibody (Catalog # P03794-2). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: P03794-2
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Phospho-Synapsin I (Ser62,67) Syn1 Antibody

View all Synapsin I Antibodies

SKU/Catalog Number

P03794-2

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-Synapsin I (Ser62, 67) Syn1 Antibody (Catalog # P03794-2). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-Synapsin I (Ser62,67) Syn1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03794-2)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser62/67 of rat synapsin, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P03794-2 is reactive to SYN1 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

52 kDa

Calculated molecular weight

74097 MW

Background of Synapsin I

Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Ser-549 along with Ser-62 and Ser-67 are the sites of Synapsin I that are phosphorylated by MAP kinase (Czernik et al., 1987; Jovanovic et al., 1996).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

P03794-2 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

Gene/Protein Information For SYN1 (Source: Uniprot.org, NCBI)

Gene Name

SYN1

Full Name

Synapsin-1

Weight

74097 MW

Superfamily

synapsin family

Alternative Names

Brain protein 4.1 antibody, SYN 1 antibody, SYN 1a antibody, SYN 1b antibody, SYN I antibody, SYN1 antibody, SYN1_HUMAN antibody, SYN1a antibody, SYN1b antibody, Synapsin 1 antibody, Synapsin I antibody, Synapsin-1 antibody, Synapsin1 antibody, SynapsinI antibody, SYNI antibody SYN1 EPILX, MRX50a, SYN1b, SYNI, SYN1 synapsin I synapsin-1|brain protein 4.1|synapsin Ib

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SYN1, check out the SYN1 Infographic

SYN1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SYN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-Phospho-Synapsin I (Ser62,67) Syn1 Antibody

Question

I am interested in to test anti-Phospho-Synapsin I (Ser62,67) antibody P03794-2 on rat anterior cingulate cortex for research purposes, then I may be interested in using anti-Phospho-Synapsin I (Ser62,67) antibody P03794-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-03-12

Answer

The products we sell, including anti-Phospho-Synapsin I (Ser62,67) antibody P03794-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-03-12

Question

We have been able to see staining in rat brain cortex. Are there any suggestions? Is anti-Phospho-Synapsin I (Ser62,67) antibody supposed to stain brain cortex positively?

Verified Customer

Verified customer

Asked: 2020-02-18

Answer

Based on literature brain cortex does express SYN1. Based on Uniprot.org, SYN1 is expressed in anterior cingulate cortex, brain, brain cortex, among other tissues. Regarding which tissues have SYN1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2110562, 15772651
Brain cortex, Pubmed ID: 15822905

Boster Scientific Support

Answered: 2020-02-18

Question

Our lab were well pleased with the WB result of your anti-Phospho-Synapsin I (Ser62,67) antibody. However we have observed positive staining in anterior cingulate cortex cell junction using this antibody. Is that expected? Could you tell me where is SYN1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-07-26

Answer

From what I have seen in literature, anterior cingulate cortex does express SYN1. Generally SYN1 expresses in cell junction, synapse. golgi apparatus. Regarding which tissues have SYN1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2110562, 15772651
Brain cortex, Pubmed ID: 15822905

Boster Scientific Support

Answered: 2019-07-26

Question

I was wanting to use your anti-Phospho-Synapsin I (Ser62,67) antibody for WB for rat anterior cingulate cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat anterior cingulate cortex identification?

Verified Customer

Verified customer

Asked: 2019-03-28

Answer

As indicated on the product datasheet, P03794-2 anti-Phospho-Synapsin I (Ser62,67) antibody has been validated for WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat anterior cingulate cortex in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-03-28

Question

We are currently using anti-Phospho-Synapsin I (Ser62,67) antibody P03794-2 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says rat. Is it likely that the antibody can work on bovine tissues as well?

S. Zhao

Verified customer

Asked: 2015-01-02

Answer

The anti-Phospho-Synapsin I (Ser62,67) antibody (P03794-2) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-01-02

Question

Is this P03794-2 anti-Phospho-Synapsin I (Ser62,67) antibody reactive to the isotypes of SYN1?

C. Jones

Verified customer

Asked: 2013-07-19

Answer

The immunogen of P03794-2 anti-Phospho-Synapsin I (Ser62,67) antibody is The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser62,67 of synapsin I. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-07-19

Order DetailsPrice
P03794-2

100uL

$481

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P03794-2
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00

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