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Product Info Summary
| SKU: | P03794-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-Synapsin I (Ser603) Syn1 Antibody
View all Synapsin I Antibodies
SKU/Catalog Number
P03794-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Synapsin I (Ser603) Syn1 Antibody (Catalog # P03794-1). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-Synapsin I (Ser603) Syn1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03794-1)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser603 of rat synapsin, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P03794-1 is reactive to SYN1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
74097 MW
Background of Synapsin I
Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogenesis regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Serine 603 is the site on synapsin I that is phosphorylated by calcium calmodulin kinase II and by p21-activated kinases (Sakurada et al., 2002; Czernik et al., 1987). Phosphorylation of this site is thought to regulate synaptic vesicle function (Nayak et al., 1996; Bahler and Greengard, 1987; McGuinness et al., 1989).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P03794-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Positive Control
Validation Images & Assay Conditions
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Western blot of rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser603 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 minutes).
Protein Target Info & Infographic
Gene/Protein Information For SYN1 (Source: Uniprot.org, NCBI)
Gene Name
SYN1
Full Name
Synapsin-1
Weight
74097 MW
Superfamily
synapsin family
Alternative Names
SYN1; SYN1a; SYN1b; Synapsin 1; Synapsin I; synapsin IBrain protein 4.1; synapsin-1; synaptic marker; synaptic markers immunohistochemistry; SYNI SYN1 EPILX, MRX50a, SYN1b, SYNI, SYN1 synapsin I synapsin-1|brain protein 4.1|synapsin Ib
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYN1, check out the SYN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Synapsin I (Ser603) Syn1 Antibody (P03794-1)
Hello CJ!
P03794-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Neurotoxicity induced by zinc oxide nanoparticles: age-related differences and interaction
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Phospho-Synapsin I (Ser603) Syn1 Antibody
Question
We have been able to see staining in mouse brain cortex. Any tips? Is anti-Phospho-Synapsin I (Ser603) antibody supposed to stain brain cortex positively?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
According to literature brain cortex does express SYN1. According to Uniprot.org, SYN1 is expressed in anterior cingulate cortex, brain, brain cortex, among other tissues. Regarding which tissues have SYN1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2110562, 15772651
Brain cortex, Pubmed ID: 15822905
Boster Scientific Support
Answered: 2019-09-10
Question
Our team were well pleased with the WB result of your anti-Phospho-Synapsin I (Ser603) antibody. However we have observed positive staining in brain synapse. golgi apparatus using this antibody. Is that expected? Could you tell me where is SYN1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
According to literature, brain does express SYN1. Generally SYN1 expresses in cell junction, synapse. golgi apparatus. Regarding which tissues have SYN1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2110562, 15772651
Brain cortex, Pubmed ID: 15822905
Boster Scientific Support
Answered: 2019-08-02
Question
We are currently using anti-Phospho-Synapsin I (Ser603) antibody P03794-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
The anti-Phospho-Synapsin I (Ser603) antibody (P03794-1) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-11
