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Product Info Summary
| SKU: | A00110T453-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-Sp1 (T453) Antibody
SKU/Catalog Number
A00110T453-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Sp1 (T453) Antibody catalog # A00110T453-1. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Sp1 (T453) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00110T453-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human SP1 around the phosphorylation site of Thr453. AA range:421-470
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00110T453-1 is reactive to SP1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
80693 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00110T453-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Immunofluorescence analysis of HeLa cells, using SP1 (Phospho-Thr453) antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain, using SP1 (Phospho-Thr453) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from A549 cells, using SP1 (Phospho-Thr453) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For SP1 (Source: Uniprot.org, NCBI)
Gene Name
SP1
Full Name
Transcription factor Sp1
Weight
80693 MW
Superfamily
Sp1 C2H2-type zinc-finger protein family
Alternative Names
Transcription factor Sp1;SP1;TSFP1; SP1 Sp1 transcription factor transcription factor Sp1|specificity protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SP1, check out the SP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Sp1 (T453) Antibody (A00110T453-1)
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