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Product Info Summary
| SKU: | A13222 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-LRFN1 Antibody
View all SALM2/LRFN1 Antibodies
SKU/Catalog Number
A13222
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LRFN1 Antibody (Catalog # A13222). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
LRFN1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-LRFN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13222)
Host
Rabbit
Contents
LRFN1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
LRFN1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human LRFN1. The immunogen is located within amino acids 700 - 750 of LRFN1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody is predicted to not cross-react with other members of the LRFN family.
Reactive Species
A13222 is reactive to LRFN1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
82318 MW
Background of SALM2/LRFN1
LRFN1 is one of a family of five transmembrane glycoproteins that are highly expressed in neuronal tissues. LRFN proteins share leucine-rich repeat (LRR)-immunoglobulin-like (Ig)-fibronectin type III (Fn)-transmembrane domain structure with other members of the LRR-Ig-Fn protein superfamily such as the Slitrk family of proteins. Expression of LRFN1, -3, and -4 mRNA was detected in embryonic neuronal cells, while Lrfn2 and Lrfn5 expression was primarily restricted to more mature cells. LRFN1, -2, and -4 bound to PDZ domains of postsynaptic PSD95, re-distributing PSD95 to the cell periphery. It has been suggested that the Lrfn proteins play a role in the developing and/or mature vertebrate nervous system. At least two isoforms of LRFN1 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13222 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
LRFN1 antibody can be used for detection of LRFN1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of LRFN1 in human brain lysate with LRFN1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of LRFN1 in mouse brain tissue with LRFN1 antibody at 2.5 μg/mL.
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Immunofluorescence of LRFN1 in mouse brain tissue with LRFN1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For LRFN1 (Source: Uniprot.org, NCBI)
Gene Name
LRFN1
Full Name
Leucine-rich repeat and fibronectin type III domain-containing protein 1
Weight
82318 MW
Superfamily
LRFN family
Alternative Names
SALM2, KIAA1484, SALM2, Leucine-rich repeat and fibronectin type III domain-containing protein 1, Synaptic adhesion-like molecule 2 LRFN1 SALM2 leucine rich repeat and fibronectin type III domain containing 1 leucine-rich repeat and fibronectin type III domain-containing protein 1|CTC-246B18.8|synaptic adhesion-like molecule 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LRFN1, check out the LRFN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LRFN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LRFN1 Antibody (A13222)
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