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Product Info Summary
| SKU: | A00293Y1015 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Phospho-Ret (Y1015) Antibody
SKU/Catalog Number
A00293Y1015
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Ret (Y1015) Antibody catalog # A00293Y1015. Tested in ELISA, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Ret (Y1015) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00293Y1015)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Ret around the phosphorylation site of Tyr1015. AA range:991-1040
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00293Y1015 is reactive to RET in Human, Monkey, Mouse, Rat
Reconstitution
Calculated molecular weight
124319 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00293Y1015 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:5000
Validation Images & Assay Conditions
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Ret (Phospho-Tyr1015) antibody
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Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using Ret (Phospho-Tyr1015) antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Protein Target Info & Infographic
Gene/Protein Information For RET (Source: Uniprot.org, NCBI)
Gene Name
RET
Full Name
Proto-oncogene tyrosine-protein kinase receptor Ret
Weight
124319 MW
Superfamily
protein kinase superfamily
Alternative Names
Proto-oncogene tyrosine-protein kinase receptor Ret ;2.7.10.1;Cadherin family member 12;Proto-oncogene c-Ret;Soluble RET kinase fragment;Extracellular cell-membrane anchored RET cadherin 120 kDa fragment;RET RET CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC-ELE1, RET ret proto-oncogene proto-oncogene tyrosine-protein kinase receptor Ret|RET receptor tyrosine kinase|cadherin family member 12|cadherin-related family member 16|proto-oncogene c-Ret|rearranged during transfection|ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RET, check out the RET Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RET: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Ret (Y1015) Antibody (A00293Y1015)
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