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Product Info Summary
| SKU: | A00075T599 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-Phospho-Raf-B (T599) BRAF Antibody
SKU/Catalog Number
A00075T599
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Raf-B (T599) BRAF Antibody catalog # A00075T599. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Raf-B (T599) BRAF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00075T599)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human B-RAF around the phosphorylation site of Thr599. AA range:571-620
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00075T599 is reactive to BRAF in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
84437 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00075T599 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100-1:300
ELISA 1:40000
Validation Images & Assay Conditions
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using B-RAF (Phospho-Thr599) antibody
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using B-RAF (Phospho-Thr599) antibody. The picture on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For BRAF (Source: Uniprot.org, NCBI)
Gene Name
BRAF
Full Name
Serine/threonine-protein kinase B-raf
Weight
84437 MW
Superfamily
protein kinase superfamily
Alternative Names
Serine/threonine-protein kinase B-raf;2.7.11.1;Proto-oncogene B-Raf;p94;v-Raf murine sarcoma viral oncogene homolog B1;BRAF;BRAF1, RAFB1; BRAF B-RAF1, B-raf1, NS7, RAFB1, BRAF B-Raf proto-oncogene, serine/threonine kinase serine/threonine-protein kinase B-raf|94 kDa B-raf protein|B-Raf proto-oncogene serine/threonine-protein kinase (p94)|B-Raf serine/threonine-protein|murine sarcoma viral (v-raf) oncogene homolog B1|proto-oncogene B-Raf|v-raf murine sarcoma viral oncogene homolog B|v-raf murine sarcoma viral oncogene homolog B1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BRAF, check out the BRAF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BRAF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Raf-B (T599) BRAF Antibody (A00075T599)
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