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Product Info Summary
| SKU: | PA1848 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-B Raf/BRAF Antibody Picoband®
SKU/Catalog Number
PA1848
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-B Raf/BRAF Antibody catalog # PA1848. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-B Raf/BRAF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1848)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human B Raf, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1848 is reactive to BRAF in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
46 kDa
Calculated molecular weight
84437 MW
Background of B-Raf
BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B-Raf, also known as ONCOGENE BRAF, BRAF1, RAFB1, Proto-oncogene B-Raf p94, v-Raf murine sarcoma viral oncogene homolog B1, proto-oncogene B-Raf, v-Raf murine sarcoma viral oncogene homolog B1, while the protein is more formally known as serine/threonine-protein kinase B-Raf. BRAF deduced 651-amino acid protein has a calculated molecular mass of 72.5 kD. Northern blot analysis detected transcripts of 10 and 13 kb in cerebrum, fetal brain, and placenta and transcripts of 2.6 and 4.5 kb in testis. Testis also showed lower expression of the 10- and 13-kb transcripts.B-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1848 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Brain Tissue, Rat Testis Tissue, Rat Liver Tissue, SW620 Cell, COLO320 Cell, PC-12 Cell,
Validation Images & Assay Conditions
Click image to see more details
Anti-B Raf antibody, PA1848, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Testis Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: SW620 Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: PC-12 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For BRAF (Source: Uniprot.org, NCBI)
Gene Name
BRAF
Full Name
Serine/threonine-protein kinase B-raf
Weight
84437 MW
Superfamily
protein kinase superfamily
Alternative Names
Serine/threonine-protein kinase B-raf;2.7.11.1;Proto-oncogene B-Raf;p94;v-Raf murine sarcoma viral oncogene homolog B1;BRAF;BRAF1, RAFB1; BRAF B-RAF1, B-raf1, NS7, RAFB1, BRAF B-Raf proto-oncogene, serine/threonine kinase serine/threonine-protein kinase B-raf|94 kDa B-raf protein|B-Raf proto-oncogene serine/threonine-protein kinase (p94)|B-Raf serine/threonine-protein|murine sarcoma viral (v-raf) oncogene homolog B1|proto-oncogene B-Raf|v-raf murine sarcoma viral oncogene homolog B|v-raf murine sarcoma viral oncogene homolog B1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BRAF, check out the BRAF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BRAF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-B Raf/BRAF Antibody Picoband® (PA1848)
Hello CJ!
PA1848 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Feng,S.,Wang,S.,Sun,S.,Su,H.,& Zhang,L.(2021).Effects of combination treatment with transcranial magnetic stimulation and bone marrow mesenchymal stem cell transplantation or Raf inhibition on spinal cord injury in rats.Molecular Medicine Reports,23,294.h
Species: Rat
PA1848 usage in article: APP:WB, SAMPLE:SPINAL CORD TISSUE, DILUTION:1:1000
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-B Raf/BRAF Antibody Picoband®
Question
Is there a BSA free version of anti-B Raf/BRAF antibody PA1848 available?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-B Raf/BRAF antibody PA1848 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-08
Question
I see that the anti-B Raf/BRAF antibody PA1848 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-10-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-10-25
Question
I was wanting to use your anti-B Raf/BRAF antibody for WB for mouse colon carcinoma hepatoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse colon carcinoma hepatoma identification?
G. Mitchell
Verified customer
Asked: 2017-08-01
Answer
You can see on the product datasheet, PA1848 anti-B Raf/BRAF antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse colon carcinoma hepatoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-01
Question
Will PA1848 anti-B Raf/BRAF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Jha
Verified customer
Asked: 2013-11-13
Answer
You can see on the product datasheet, PA1848 anti-B Raf/BRAF antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-11-13
