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Product Info Summary
| SKU: | EK1369 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine®
SKU/Catalog Number
EK1369
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine™ (96 Tests). Quantitate Human HJV in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1369)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q36-D400
Sensitivity
<15 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1369 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1369 is reactive to Hjv in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1369 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Hjv
Hemojuvelin (HJV/RGMc/HFE2) is a membrane-bound and soluble protein in mammals. It is a member of the repulsive guidance molecule family of proteins. In humans, the hemojuvelin protein is encoded by the HFE2 gene. The product of this gene is involved in iron metabolism. It may be a component of the signaling pathway which activates hepcidin or it may act as a modulator of hepcidin expression. It could also represent the cellular receptor for hepcidin. Two uORFs in the 5' UTR negatively regulate the expression and activity of the encoded protein. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Defects in this gene are the cause of hemochromatosis type 2A, also called juvenile hemochromatosis (JH). JH is an early-onset autosomal recessive disorder due to severe iron overload resulting in hypogonadotrophic hypogonadism, hepatic fibrosis or cirrhosis and cardiomyopathy, occurring typically before age of 30.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1369-CAP | Anti-Human Hjv Pre-coated 96-well strip microplate | 1 |
| EK1369-ST | Human Hjv Standard | 2 vials, 10 ng/tube |
| EK1369-DA | Human Hjv Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.102 | 0.233 | 0.192 | 0.284 | 0.482 | 0.773 | 1.266 | 1.981 |
Data Example Images
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Human Hemojuvelin/RGM-C PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Hjv, Dilution ratio of 1:1, concentration in serum and plasma is 0-400 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 285 | 1139 | 5792 | 1123 | 1123 | 5755 |
| Standard deviation | 17.38 | 72.89 | 457.56 | 18.45 | 74.11 | 552.48 |
| CV (%) | 6.1% | 6.4% | 7.9% | 6.6% | 6.6% | 9.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Hjv in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 285 | 292 | 319 | 286 | 295 | 13.82 | 4.6% |
| Sample 2 | 1139 | 1247 | 1212 | 1220 | 1204 | 39.97 | 3.3% |
| Sample 3 | 5792 | 6174 | 5259 | 6098 | 5830 | 359.73 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For Hjv (Source: Uniprot.Org, NCBI)
Gene Name
Hjv
Full Name
Hemojuvelin
Weight
45080 MW
Superfamily
repulsive guidance molecule (RGM) family
Alternative Names
Hemojuvelin Hjv|2310035L15Rik, 5230400G09Rik, AI414844, AI789733, DL-, DL-M, HJ, Hfe2, Rgm, Rgmc, hemoju, hemojuvelin|hemojuvelin BMP co-receptor|hemojuvelin|RGM domain family member C|hemochromatosis type 2 (juvenile) (human homolog)|hemochromatosis type 2 protein homolog|hemojuvelin BMP coreceptor|repulsive guidance molecule C
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Hjv, check out the Hjv Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Hjv: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine® (EK1369)
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Customer Q&As
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5 Customer Q&As for Human Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
H. Evans
Verified customer
Asked: 2019-09-25
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-09-25
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-03-26
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring HFE2 using the EK1369 Human HFE2 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-03-17
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2019-03-17
Question
Q: What is the optimal O.D. value for HFE2 ELISA kit? I used your HFE2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain HFE2 even though the O.D. values are not very high?
W. Mehta
Verified customer
Asked: 2018-01-21
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this HFE2 ELISA kit to have sensitivity of 15pg/ml, that means the minimum amount of HFE2 that can be declared/interpreted as positive by the above standard is 15pg/ml.
Boster Scientific Support
Answered: 2018-01-21
Question
Q: how to analyze ELISA data? I measured HFE2 level in cell lysates.
Y. Harris
Verified customer
Asked: 2016-07-10
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-07-10
