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Product Info Summary
| SKU: | A00743Y657 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Phospho-PKC alpha (Y657) PRKCA Antibody
SKU/Catalog Number
A00743Y657
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-PKC alpha (Y657) PRKCA Antibody catalog # A00743Y657. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-PKC alpha (Y657) PRKCA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00743Y657)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PKC alpha around the phosphorylation site of Tyr657. AA range:623-672
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00743Y657 is reactive to PRKCA in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
76750 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00743Y657 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of 293 cells using Phospho-PKC α (Y657) polyclonal antibody diluted at 1:1000
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Western blot analysis of lysates from COLO205 cells, using PKC alpha (Phospho-Tyr657) antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Protein Target Info & Infographic
Gene/Protein Information For PRKCA (Source: Uniprot.org, NCBI)
Gene Name
PRKCA
Full Name
Protein kinase C alpha type
Weight
76750 MW
Superfamily
protein kinase superfamily
Alternative Names
Protein kinase C alpha type;PKC-A;PKC-alpha;2.7.11.13;PRKCA;PKCA, PRKACA; PRKCA AAG6, PKC-alpha, PKCA, PKCI+/-, PKCalpha, PRKACA protein kinase C alpha protein kinase C alpha type|PKC-A|aging-associated gene 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKCA, check out the PRKCA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKCA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-PKC alpha (Y657) PRKCA Antibody (A00743Y657)
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