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Product Info Summary
| SKU: | P05977 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-PFK-2 car (Ser483) PFKFB2 Antibody
SKU/Catalog Number
P05977
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-PFK-2 car (Ser483) PFKFB2 Antibody (Catalog # P05977). Tested in WB, IHC, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-PFK-2 car (Ser483) PFKFB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P05977)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Immunogen
Synthesized peptide derived from human PFK-2 car around the phosphorylation site of S483.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P05977 is reactive to PFKFB2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
58477 MW
Background of PFKFB2
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
Ferrer, et al. (2003) Neuropathology & Applied Neurobiology 29: 23
Zhonghong Guan, et al. (1999) J Biol Chem, Vol. 274: 36200-36206
D.Margriet Ouwens1, et al. (2002)The EMBO Journal 21: 3782
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P05977 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1/500 - 1/2000
Immunohistochemistry: 1/100 - 1/300
ELISA: 1/40000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western Blot analysis of PC3 cells using Phospho-PFK-2 car (S483) Polyclonal antibody
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Western Blot analysis of Hela cells using Phospho-PFK-2 car (S483) Polyclonal antibody
Protein Target Info & Infographic
Gene/Protein Information For PFKFB2 (Source: Uniprot.org, NCBI)
Gene Name
PFKFB2
Full Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2
Weight
58477 MW
Alternative Names
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2;6PF-2-K/Fru-2,6-P2ase 2;PFK/FBPase 2;6PF-2-K/Fru-2,6-P2ase heart-type isozyme;6-phosphofructo-2-kinase;2.7.1.105;Fructose-2,6-bisphosphatase;3.1.3.46;PFKFB2; PFKFB2 PFK-2/FBPase-2 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2|6PF-2-K/Fru-2,6-P2ASE heart-type isozyme|6PF-2-K/Fru-2,6-P2ase 2|PFK/FBPase 2|PFKFB, cardiac|fructose-2,6-bisphosphatase, cardiac isozyme
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PFKFB2, check out the PFKFB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PFKFB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-PFK-2 car (Ser483) PFKFB2 Antibody (P05977)
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No publications found for P05977
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Phospho-PFK-2 car (Ser483) PFKFB2 Antibody
Question
I was wanting to use your anti-Phospho-PFK-2 car (Ser483) Polyclonal antibody for WB for rat trachea on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat trachea identification?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
You can see on the product datasheet, P05977 anti-Phospho-PFK-2 car (Ser483) Polyclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat trachea in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-29
Question
Can you help my question with product P05977, anti-Phospho-PFK-2 car (Ser483) Polyclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P05977 anti-Phospho-PFK-2 car (Ser483) Polyclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-17
Question
Is this P05977 anti-Phospho-PFK-2 car (Ser483) Polyclonal antibody reactive to the isotypes of PFKFB2?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
The immunogen of P05977 anti-Phospho-PFK-2 car (Ser483) Polyclonal antibody is Synthesized peptide derived from human PFK-2 car around the phosphorylation site of S483. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-30
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for trachea using anti-Phospho-PFK-2 car (Ser483) Polyclonal antibody P05977. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-11
Question
Will P05977 anti-Phospho-PFK-2 car (Ser483) Polyclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-31
Answer
You can see on the product datasheet, P05977 anti-Phospho-PFK-2 car (Ser483) Polyclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-31
Question
I see that the anti-Phospho-PFK-2 car (Ser483) Polyclonal antibody P05977 works with WB, what is the protocol used to produce the result images on the product page?
B. Li
Verified customer
Asked: 2017-04-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-04-28
Question
Is a blocking peptide available for product anti-Phospho-PFK-2 car (Ser483) Polyclonal antibody (P05977)?
H. Parker
Verified customer
Asked: 2014-12-24
Answer
We do provide the blocking peptide for product anti-Phospho-PFK-2 car (Ser483) Polyclonal antibody (P05977). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-12-24
