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Product Info Summary
| SKU: | A04133-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-MARCKS (Ab-158) Antibody
SKU/Catalog Number
A04133-2
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MARCKS (Ab-158) Antibody (Catalog # A04133-2). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MARCKS (Ab-158) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04133-2)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.156~160 (R-F-S-F-K) derived from Human MARCKS.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04133-2 is reactive to MARCKS in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
49308 MW
Background of MARCKS
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30; 102(35): 12407-12412
Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26; 280(3): 605-609
Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15; 359(2): 151-159
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04133-2 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 80kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MARCKS using anti-MARCKS antibody (A04133-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARCKS antigen affinity purified polyclonal antibody (Catalog # A04133-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for MARCKS.
Click image to see more details
Immunofluorescence staining of methanol-fixed Hela cells using MARCKS(Ab-158) Antibody #A04133-2.
Protein Target Info & Infographic
Gene/Protein Information For MARCKS (Source: Uniprot.org, NCBI)
Gene Name
MARCKS
Full Name
Myristoylated alanine-rich C-kinase substrate
Weight
49308 MW
Superfamily
MARCKS family
Alternative Names
Serine/threonine-protein kinase 24;2.7.11.1;Mammalian STE20-like protein kinase 3;MST-3;STE20-like kinase MST3;Serine/threonine-protein kinase 24 36 kDa subunit;Mammalian STE20-like protein kinase 3 N-terminal;MST3/N;Serine/threonine-protein kinase 24 12 kDa subunit;Mammalian STE20-like protein kinase 3 C-terminal;MST3/C;STK24;MST3, STK3; MARCKS 80K-L, MACS, PKCSL, PRKCSL myristoylated alanine rich protein kinase C substrate myristoylated alanine-rich C-kinase substrate|myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L)|phosphomyristin|protein kinase C substrate, 80 kDa protein, light chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MARCKS, check out the MARCKS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MARCKS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MARCKS (Ab-158) Antibody (A04133-2)
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No publications found for A04133-2
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Customer Q&As
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13 Customer Q&As for Anti-MARCKS (Ab-158) Antibody
Question
Is a blocking peptide available for product anti-MARCKS (Ab-158) antibody (A04133-2)?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
We do provide the blocking peptide for product anti-MARCKS (Ab-158) antibody (A04133-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-16
Question
We are currently using anti-MARCKS (Ab-158) antibody A04133-2 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
The anti-MARCKS (Ab-158) antibody (A04133-2) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-03
Question
Does anti-MARCKS (Ab-158) antibody A04133-2 work for WB with erythroleukemia?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
According to the expression profile of erythroleukemia, MARCKS is highly expressed in erythroleukemia. So, it is likely that anti-MARCKS (Ab-158) antibody A04133-2 will work for WB with erythroleukemia.
Boster Scientific Support
Answered: 2020-01-14
Question
Do you have a BSA free version of anti-MARCKS (Ab-158) antibody A04133-2 available?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MARCKS (Ab-158) antibody A04133-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-26
Question
I was wanting to use your anti-MARCKS (Ab-158) antibody for WB for human erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
It shows on the product datasheet, A04133-2 anti-MARCKS (Ab-158) antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-15
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-MARCKS (Ab-158) antibody A04133-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-03
Question
Is this A04133-2 anti-MARCKS (Ab-158) antibody reactive to the isotypes of MARCKS?
Verified Customer
Verified customer
Asked: 2019-01-09
Answer
The immunogen of A04133-2 anti-MARCKS (Ab-158) antibody is Peptide sequence around aa.156~160 (R-F-S-F-K) derived from Human MARCKS. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-09
Question
Will anti-MARCKS (Ab-158) antibody A04133-2 work on pig IF with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-01-02
Answer
Our lab technicians have not tested anti-MARCKS (Ab-158) antibody A04133-2 on pig. You can run a BLAST between pig and the immunogen sequence of anti-MARCKS (Ab-158) antibody A04133-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-02
Question
My question regards to test anti-MARCKS (Ab-158) antibody A04133-2 on human erythroleukemia for research purposes, then I may be interested in using anti-MARCKS (Ab-158) antibody A04133-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Carter
Verified customer
Asked: 2018-07-24
Answer
The products we sell, including anti-MARCKS (Ab-158) antibody A04133-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-24
Question
My question regarding product A04133-2, anti-MARCKS (Ab-158) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-05-04
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04133-2 anti-MARCKS (Ab-158) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-04
Question
Please see the WB image, lot number and protocol we used for erythroleukemia using anti-MARCKS (Ab-158) antibody A04133-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-09-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-21
Question
Would A04133-2 anti-MARCKS (Ab-158) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Thomas
Verified customer
Asked: 2016-08-02
Answer
It shows on the product datasheet, A04133-2 anti-MARCKS (Ab-158) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-08-02
Question
I see that the anti-MARCKS (Ab-158) antibody A04133-2 works with WB, what is the protocol used to produce the result images on the product page?
J. Patel
Verified customer
Asked: 2014-02-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-02-28
