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Product Info Summary
| SKU: | A00795T224 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-KIR6.2 (T224) KCNJ11 Antibody
SKU/Catalog Number
A00795T224
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-KIR6.2 (T224) KCNJ11 Antibody catalog # A00795T224. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-KIR6.2 (T224) KCNJ11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00795T224)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Kir6.2 around the phosphorylation site of Thr224. AA range:190-239
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00795T224 is reactive to KCNJ11 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
43541 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00795T224 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human brain. antibody was diluted at 1:100 (4° overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunofluorescence analysis of HUVEC cells, using Kir6.2 (Phospho-Thr224) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from HeLa cells, using Kir6.2 (Phospho-Thr224) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For KCNJ11 (Source: Uniprot.org, NCBI)
Gene Name
KCNJ11
Full Name
ATP-sensitive inward rectifier potassium channel 11
Weight
43541 MW
Superfamily
inward rectifier-type potassium channel (TC 1.A.2.1) family
Alternative Names
ATP-sensitive inward rectifier potassium channel 11;IKATP;Inward rectifier K (+) channel Kir6.2;Potassium channel, inwardly rectifying subfamily J member 11;KCNJ11; KCNJ11 BIR, HHF2, IKATP, KIR6.2, MODY13, PHHI, PNDM2, TNDM3 potassium inwardly rectifying channel subfamily J member 11 ATP-sensitive inward rectifier potassium channel 11|beta-cell inward rectifier subunit|inward rectifier K(+) channel Kir6.2|inwardly rectifing potassium channel subfamily J member 11|inwardly rectifying potassium channel KIR6.2|inwardly-rectifying potassium channel subfamily J member 11|potassium channel inwardly rectifing subfamily J member 11|potassium channel, inwardly rectifying subfamily J member 11|potassium voltage-gated channel subfamily J member 11
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KCNJ11, check out the KCNJ11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KCNJ11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-KIR6.2 (T224) KCNJ11 Antibody (A00795T224)
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