Product Info Summary
SKU: | A00330Y1022-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-JAK1 (Y1022) Antibody
SKU/Catalog Number
A00330Y1022-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-JAK1 (Y1022) Antibody catalog # A00330Y1022-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-JAK1 (Y1022) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00330Y1022-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human JAK1 around the phosphorylation site of Y1022.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00330Y1022-1 is reactive to JAK1 in Human, Mouse, Rat
Applications
A00330Y1022-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
133277 MW
Background of JAK1
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
Patricia Giraldo, Nucleic Acids Res., Nov 2003; 31: 6290 - 6305.
R Halaban, J. Cell Biol., Aug 1983; 97: 480.
Hee-Young Park, J. Biol. Chem., Jun 1999; 274: 16470.
BB Fuller, J. Biol. Chem., Mar 1987; 262: 4024 - 4033.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 10. Immunohistochemistry validation of JAK1 using Anti-Phospho-JAK1 (Y1022) Antibody (A00330Y1022-1).
Immunohistochemical analysis of mouse heart tissue. A00330Y1022-1 was diluted at 1:200 (4°CClick image to see more details
Figure 11. Immunohistochemistry validation of JAK1 using Anti-Phospho-JAK1 (Y1022) Antibody (A00330Y1022-1).
Immunohistochemical analysis of mouse kidney tissue. A00330Y1022-1 was diluted at 1:200 (4°CClick image to see more details
Figure 12. Immunohistochemistry validation of JAK1 using Anti-Phospho-JAK1 (Y1022) Antibody (A00330Y1022-1).
Immunohistochemical analysis of mouse liver tissue. A00330Y1022-1 was diluted at 1:200 (4°CClick image to see more details
Figure 13. Immunohistochemistry validation of JAK1 using Anti-Phospho-JAK1 (Y1022) Antibody (A00330Y1022-1).
Immunohistochemical analysis of mouse lung tissue. A00330Y1022-1 was diluted at 1:200 (4°CClick image to see more details
Figure 14. Immunohistochemistry validation of JAK1 using Anti-Phospho-JAK1 (Y1022) Antibody (A00330Y1022-1).
Immunohistochemical analysis of mouse spleen tissue. A00330Y1022-1 was diluted at 1:200 (4°CClick image to see more details
Figure 15. Immunohistochemistry validation of JAK1 using Anti-Phospho-JAK1 (Y1022) Antibody (A00330Y1022-1).
Immunohistochemical analysis of rat brain tissue. A00330Y1022-1 was diluted at 1:200 (4°CClick image to see more details
Figure 16. Immunohistochemistry validation of JAK1 using Anti-Phospho-JAK1 (Y1022) Antibody (A00330Y1022-1).
Immunohistochemical analysis of rat kidney tissue. A00330Y1022-1 was diluted at 1:200 (4°CClick image to see more details
Figure 17. Immunohistochemistry validation of JAK1 using Anti-Phospho-JAK1 (Y1022) Antibody (A00330Y1022-1).
Immunohistochemical analysis of rat lung tissue. Anti-Cleaved-Caspase-1 (D210) antibody was diluted at 1:200 (4°CClick image to see more details
Figure 18. Immunohistochemistry validation of JAK1 using Anti-Phospho-JAK1 (Y1022) Antibody (A00330Y1022-1).
Immunohistochemical analysis of rat spleen tissue. Anti-Cleaved-Caspase-1 (D210) antibody was diluted at 1:200 (4°CClick image to see more details
Figure 2. Immunohistochemistry validation of JAK1 using Anti-Phospho-JAK1 (Y1022) Antibody (A00330Y1022-1).
Immunohistochemical analysis of human-appendix tissue. Anti-Phospho-JAK1 (Y1022) at 1:200 (4°CClick image to see more details
Figure 3. Immunohistochemistry validation of JAK1 using Anti-Phospho-JAK1 (Y1022) Antibody (A00330Y1022-1).
Immunohistochemical analysis of human colon tissue. Anti-Phospho-JAK1 (Y1022) at 1:200 (4°CClick image to see more details
Figure 4. Immunohistochemistry validation of JAK1 using Anti-Phospho-JAK1 (Y1022) Antibody (A00330Y1022-1).
Immunohistochemical analysis of human liver cancer tissue. Anti-Phospho-JAK1 (Y1022) at 1:200 (4°CClick image to see more details
Figure 5. Immunohistochemistry validation of JAK1 using Anti-Phospho-JAK1 (Y1022) Antibody (A00330Y1022-1).
Immunohistochemical analysis of human liver tissue. Anti-Phospho-JAK1 (Y1022) at 1:200 (4°CClick image to see more details
Figure 6. Immunohistochemistry validation of JAK1 using Anti-Phospho-JAK1 (Y1022) Antibody (A00330Y1022-1).
Immunohistochemical analysis of human lung cancer tissue. Anti-Phospho-JAK1 (Y1022) at 1:200 (4°CClick image to see more details
Figure 7. Immunohistochemistry validation of JAK1 using Anti-Phospho-JAK1 (Y1022) Antibody (A00330Y1022-1).
Immunohistochemical analysis of human lung tissue. Anti-Phospho-JAK1 (Y1022) at 1:200 (4°CClick image to see more details
Figure 8. Immunohistochemistry validation of JAK1 using Anti-Phospho-JAK1 (Y1022) Antibody (A00330Y1022-1).
Immunohistochemical analysis of human stomach cancer tissue. A00330Y1022-1 was diluted at 1:200 (4°CClick image to see more details
Figure 9. Immunohistochemistry validation of JAK1 using Anti-Phospho-JAK1 (Y1022) Antibody (A00330Y1022-1).
Immunohistochemical analysis of mouse colon tissue. A00330Y1022-1 was diluted at 1:200 (4°CClick image to see more details
Figure 1. Western blotting validation for Anti-Phospho-JAK1 (Y1022) Antibody A00330Y1022-1
Western blot (WB) analysis of JAK1 (phospho Tyr1022) polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For JAK1 (Source: Uniprot.org, NCBI)
Gene Name
JAK1
Full Name
Tyrosine-protein kinase JAK1
Weight
133277 MW
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.10; Jak1; JAK-1; JAK1Atyrosine-protein kinase JAK1; JAK1B; Janus kinase 1EC 2.7.10.2; JTK3 JAK1 AIIDEA, JAK1B, JTK3, JAK1 Janus kinase 1 tyrosine-protein kinase JAK1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on JAK1, check out the JAK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for JAK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-JAK1 (Y1022) Antibody (A00330Y1022-1)
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