Product Info Summary
SKU: | A00330Y1022-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-JAK1 (Y1022) Antibody
SKU/Catalog Number
A00330Y1022-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-JAK1 (Y1022) Antibody catalog # A00330Y1022-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-JAK1 (Y1022) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00330Y1022-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human JAK1 around the phosphorylation site of Tyr1022. AA range:988-1037
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00330Y1022-1 is reactive to JAK1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
133277 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00330Y1022-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescence analysis of mouse-liver tissue. 1, JAK1 (Phospho- Tyr1022) polyclonal antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Immunofluorescence analysis of mouse-liver tissue. 1, JAK1 (Phospho- Tyr1022) polyclonal antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, JAK1 (Phospho- Tyr1022) polyclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1, JAK1 (Phospho- Tyr1022) polyclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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Western Blot analysis of various cells using Phospho-JAK1 (Y1022) polyclonal antibody diluted at 1:500
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using JAK1 (Phospho-Tyr1022) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from A549 cells , using JAK1 (Phospho-Tyr1022) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For JAK1 (Source: Uniprot.org, NCBI)
Gene Name
JAK1
Full Name
Tyrosine-protein kinase JAK1
Weight
133277 MW
Superfamily
protein kinase superfamily
Alternative Names
Tyrosine-protein kinase JAK1;2.7.10.2 JAK1 AIIDEA, JAK1B, JTK3, JAK1 Janus kinase 1 tyrosine-protein kinase JAK1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on JAK1, check out the JAK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for JAK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-JAK1 (Y1022) Antibody (A00330Y1022-1)
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