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Product Info Summary
| SKU: | A12588 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-IRX1 Antibody
SKU/Catalog Number
A12588
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-IRX1 Antibody (Catalog # A12588). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRX1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12588)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human IRX1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12588 is reactive to IRX1 in Human, Mouse
Reconstitution
Calculated molecular weight
49621 MW
Background of Irx1
a member of the Iroquois homeobox protein family. Homeobox genes in this family are involved in pattern formation in the embryo. The gene product has been identified as a tumor suppressor in gastric (PMID: 21602894, 20440264) and head and neck cancers (PMID: 18559491). A pseudogene of this gene is located on chromosome 13.
Hansen L., Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Lewis M.T., Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12588 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from LOVO cells, using IRX1 antibody A12588.
Protein Target Info & Infographic
Gene/Protein Information For IRX1 (Source: Uniprot.org, NCBI)
Gene Name
IRX1
Full Name
Iroquois-class homeodomain protein IRX-1
Weight
49621 MW
Superfamily
TALE/IRO homeobox family
Alternative Names
Iroquois-class homeodomain protein IRX-1;Homeodomain protein IRXA1;Iroquois homeobox protein 1;IRX1;IRXA1; Irx1|Iroquois homeobox 1|iroquois-class homeodomain protein IRX-1|Iroquois related homeobox 1|homeodomain protein IRXA1|iroquois homeobox protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRX1, check out the IRX1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRX1 Antibody (A12588)
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No publications found for A12588
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-IRX1 Antibody
Question
We are currently using anti-IRX1 antibody A12588 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-29
Answer
The anti-IRX1 antibody (A12588) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-29
Question
Does anti-IRX1 antibody A12588 work on dog WB with metanephros?
Verified Customer
Verified customer
Asked: 2018-01-19
Answer
Our lab technicians have not tested anti-IRX1 antibody A12588 on dog. You can run a BLAST between dog and the immunogen sequence of anti-IRX1 antibody A12588 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog metanephros in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-19
Question
My question regarding product A12588, anti-IRX1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-05-23
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12588 anti-IRX1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-23
