Product Info Summary
SKU: | P00683 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Phospho-IRE1 (S724) ERN1 Rabbit Monoclonal Antibody
View all IRE1 alpha Antibodies
SKU/Catalog Number
P00683
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-IRE1 (S724) ERN1 Rabbit Monoclonal Antibody catalog # P00683. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-IRE1 (S724) ERN1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00683)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EGH-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-IRE1 (S724)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00683 is reactive to ERN1 in Human
Applications
P00683 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
185 kDa
Calculated molecular weight
109735 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
![p00683 wb7 p00683 wb7](https://www.bosterbio.com/media/catalog/product/p/0/p00683-wb7.jpg)
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
![p00683 wb8 p00683 wb8](https://www.bosterbio.com/media/catalog/product/p/0/p00683-wb8.jpg)
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
![p00683 wb p00683 wb](https://www.bosterbio.com/media/catalog/product/p/0/p00683-wb.jpg)
Click image to see more details
Western blot analysis of Phospho-IRE1 (S724) expression in (1) K562 cell lysate treated with AP; (2) Untreated K562 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For ERN1 (Source: Uniprot.org, NCBI)
Gene Name
ERN1
Full Name
Serine/threonine-protein kinase/endoribonuclease IRE1
Weight
109735 MW
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; ER to nucleus signalling 1; FLJ30999; hIRE1p; inositol-requiring 1; Inositol-requiring protein 1; IRE 1a; ire1 alpha p Ser724; ire1 alpha phosphorylation; IRE1a; ire1-alpha; IRE1MGC163279; IRE1P; MGC163277; phospho ire1 alpha; phospho-ire1 alpha; phospho-ire1a; p-ire1 alpha; protein kinase/endoribonuclease; serine/threonine-protein kinase/endoribonuclease IRE1 ERN1 IRE1, IRE1P, IRE1a, hIRE1p endoplasmic reticulum to nucleus signaling 1 serine/threonine-protein kinase/endoribonuclease IRE1|ER to nucleus signalling 1|inositol-requiring 1|inositol-requiring enzyme 1|inositol-requiring protein 1|ire1-alpha|protein kinase/endoribonuclease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERN1, check out the ERN1 Infographic
![ERN1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-IRE1 (S724) ERN1 Rabbit Monoclonal Antibody (P00683)
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No publications found for P00683
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Phospho-IRE1 (S724) ERN1 Rabbit Monoclonal Antibody
Question
Does P00683 has any rat reactivity?
Verified customer
Asked: 2021-01-08
Answer
No, the Anti-Phospho-IRE1 (S724) ERN1 Rabbit Monoclonal Antibody (P00683) doesn't react with rat samples.
Boster Scientific Support
Answered: 2021-01-10