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Product Info Summary
| SKU: | P00791-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal Antibody
View all GSK-3 beta Antibodies
SKU/Catalog Number
P00791-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal Antibody catalog # P00791-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00791-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IFC-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-GSK3 beta (Ser9)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00791-1 is reactive to GSK3B in Human
Reconstitution
Observed Molecular Weight
185 kDa
Calculated molecular weight
46744 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00791-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: human kidney tissue
ICC/IF: HeLa cell
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunofluorescent analysis of HeLa cells treated with Calyculin A, using Phospho-GSK3 beta (Ser9) Antibody.
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Western blot analysis of GSK3 beta (phospho S9) expression in 293T cell lysates, treated with Calyculin A.
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Immunohistochemical analysis of paraffin-embedded human kidney, using Phospho-GSK3 beta (Ser9) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For GSK3B (Source: Uniprot.org, NCBI)
Gene Name
GSK3B
Full Name
Glycogen synthase kinase-3 beta
Weight
46744 MW
Superfamily
protein kinase superfamily
Alternative Names
Glycogen synthase kinase-3 beta;GSK-3 beta;2.7.11.26;Serine/threonine-protein kinase GSK3B;2.7.11.1;GSK3B; GSK3B glycogen synthase kinase 3 beta glycogen synthase kinase-3 beta|GSK-3 beta|serine/threonine-protein kinase GSK3B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GSK3B, check out the GSK3B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GSK3B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal Antibody (P00791-1)
Hello CJ!
P00791-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Shi R,Liu L,Wang F,He Y,Niu Y,Wang C,Zhang X,Zhang X,Zhang H,Chen M,Wang Y.Downregulation of cytokeratin 18 induces cellular partial EMT and stemness through increasing EpCAM expression in breast cancer.Cell Signal.2020 Dec;76:109810.doi:10.1016/j.cellsig
Species: Human
P00791-1 usage in article: APP:WB, SAMPLE:MCF-7 CELL AND MDA-MB-231 CELL, DILUTION:NA
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Customer Q&As
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1 Customer Q&As for Anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal Antibody
Question
We are currently using anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal antibody P00791-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal antibody (P00791-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-04
