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Product Info Summary
| SKU: | M00791 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GSK3 beta Rabbit Monoclonal Antibody
View all GSK-3 beta Antibodies
SKU/Catalog Number
M00791
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GSK3 beta Rabbit Monoclonal Antibody catalog # M00791. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GSK3 beta Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00791)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EB-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GSK3 beta
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00791 is reactive to GSK3B in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
52 kDa
Calculated molecular weight
46903 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00791 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:3000
IHC 1:50-1:200
ICC/IF 1:100-1:500
FC 1:200-1:500
Positive Control
IHC: Human colon cancer tissue, Human prostate cancer tissue, Human small cell lung cancer tissue, Human pancreatic cancer tissue, human colon carcinome tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of GSK3 beta expression in HEK293 cell lysate.
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human small cell lung cancer , using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human pancreatic cancer, using the Antibody at 1:400 dilution.
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Immunohistochemical analysis of paraffin-embedded Human colon cancer, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded human colon carcinome, using GSK3 beta Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis of Hela cells, using GSK3 beta Antibody .
Protein Target Info & Infographic
Gene/Protein Information For GSK3B (Source: Uniprot.org, NCBI)
Gene Name
GSK3B
Full Name
Glycogen synthase kinase-3 beta
Weight
46903 MW
Superfamily
protein kinase superfamily
Alternative Names
Glycogen synthase kinase-3 beta;GSK-3 beta;2.7.11.26;Xgsk-3 protein;gsk3b;Xgsk-3; GSK3B glycogen synthase kinase 3 beta glycogen synthase kinase-3 beta|GSK-3 beta|serine/threonine-protein kinase GSK3B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GSK3B, check out the GSK3B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GSK3B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GSK3 beta Rabbit Monoclonal Antibody (M00791)
Hello CJ!
M00791 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Shi R,Liu L,Wang F,He Y,Niu Y,Wang C,Zhang X,Zhang X,Zhang H,Chen M,Wang Y.Downregulation of cytokeratin 18 induces cellular partial EMT and stemness through increasing EpCAM expression in breast cancer.Cell Signal.2020 Dec;76:109810.doi:10.1016/j.cellsig
Species: Human
M00791 usage in article: APP:WB, SAMPLE:MCF-7 CELL AND MDA-MB-231 CELL, DILUTION:NA
GSK%u20113%u03B2 may be involved in hippocampal mossy fiber sprouting in the pentylenetetrazole%u2011kindling model
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