Anti-Phospho-G3BP-1 (Ser232) Antibody

Product Name

Anti-Phospho-G3BP-1 (Ser232) Antibody

View all G3BP1 Antibodies

SKU/Catalog Number

P02199

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-G3BP-1 (Ser232) Antibody (Catalog # P02199). Tested in WB, IHC, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-G3BP-1 (Ser232) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02199)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

Peptide sequence around phosphorylation site of serine 232 (S-S-S(p)-P-A) derived from Human G3BP-1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P02199 is reactive to G3BP1 in Human

Reconstitution

Calculated molecular weight

52164 MW

Background of G3BP1

May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.

Tao WA, et al. (2005) Nat Methods. 2(8): 591-598.
Zheng H, et al. (2005) Mol Cell Proteomics. 4(6):721-730.
Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A.101 (33): 12130-12135.
Tourrihre H, et al. (2003) J Cell Biol.160 (6): 823-831.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

P02199 is guaranteed for IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Validation Images & Assay Conditions

Gene/Protein Information For G3BP1 (Source: Uniprot.org, NCBI)

Alternative Names

Ras GTPase-activating protein-binding protein 1;G3BP-1;3.6.4.12;3.6.4.13;ATP-dependent DNA helicase VIII;hDH VIII;GAP SH3 domain-binding protein 1;G3BP1;G3BP; G3BP1 G3BP, HDH-VIII G3BP stress granule assembly factor 1 ras GTPase-activating protein-binding protein 1|ATP-dependent DNA helicase VIII|DNA helicase VIII|G3BP-1|GAP SH3 domain-binding protein 1|GAP binding protein|GTPase activating protein (SH3 domain) binding protein 1|Ras-GTPase-activating protein SH3-domain-binding protein|RasGAP-associated endoribonuclease G3BP

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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