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Product Info Summary
| SKU: | A00073S322S325 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-FoxO1/3 (S322/S325) Antibody
SKU/Catalog Number
A00073S322S325
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-FoxO1/3 (S322/S325) Antibody catalog # A00073S322S325. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-FoxO1/3 (S322/S325) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00073S322S325)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human FOXO1A/3A around the phosphorylation site of Ser322 and Ser325. AA range:291-340
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00073S322S325 is reactive to FOXO1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
69662 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00073S322S325 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Validation Images & Assay Conditions
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Western Blot analysis of 3T3 cells using Phospho-FoxO1/3 (S322/S325) polyclonal antibody
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Western blot analysis of lysates from NIH/3T3 cells treated with Wortmannin 40nM 24h, using FOXO1A/3A (Phospho-Ser322/Ser325) antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Protein Target Info & Infographic
Gene/Protein Information For FOXO1 (Source: Uniprot.org, NCBI)
Gene Name
FOXO1
Full Name
Forkhead box protein O1
Weight
69662 MW
Alternative Names
Forkhead box protein O1;Forkhead box protein O1A;Forkhead in rhabdomyosarcoma;FOXO1;FKHR, FOXO1A; FOXO1 FKH1, FKHRA, FOXO1 forkhead box O1 forkhead box protein O1|forkhead box protein O1A|forkhead, Drosophila, homolog of, in rhabdomyosarcoma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOXO1, check out the FOXO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOXO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-FoxO1/3 (S322/S325) Antibody (A00073S322S325)
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