Key=store_id
Key=entity_id, value=22613
Key=attribute_set_id, value=4
Key=type_id, value=simple
Key=sku, value=P00073
Key=has_options, value=1
Key=required_options, value=1
Key=created_at, value=2017-01-17 05:22:37
Key=updated_at, value=2024-09-26 00:48:48
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=Anti-Phospho-FKHR (Ser256) FOXO1 Antibody
Key=cj_search_index
Key=publication_count, value=1
Key=qty_in_warehouses
Key=is_recurring, value=No
Key=visibility, value=Catalog, Search
Key=tax_class_id, value=Taxable Goods
Key=featured, value=No
Key=featured_image, value=Yes
Key=fb_product, value=
Key=custom_product_page, value=No
Key=free_shipping, value=No
Key=template, value=antibodies
Key=host, value=Rabbit
Key=googleshopping_exclude, value=No
Key=yoast_keyword_score, value=-1351
Key=yoast_content_score, value=90
Key=price, value=360.0000
Key=weight, value=1.0000
Key=meta_title, value=Anti-Phospho-FKHR (Ser256) Antibody (P00073) | Bosterbio
Key=meta_description, value=Boster Bio Anti-Phospho-FKHR (Ser256) FOXO1 Antibody (Catalog # P00073). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μl in Liquid form antibody.
Key=image, value=/p/0/p00073-immunofluorescence-analysis-2.jpg
Key=small_image, value=/p/0/p00073-immunofluorescence-analysis-2.jpg
Key=thumbnail, value=/p/0/p00073-immunofluorescence-analysis-2.jpg
Key=url_key, value=anti-phospho-fkhr-ser256-antibody-p00073-boster
Key=url_path, value=anti-phospho-fkhr-ser256-antibody-p00073-boster
Key=options_container, value=Product Info Column
Key=image_label, value=Immunofluorescence staining of methanol-fixed MCF7 cells using FKHR(Phospho-Ser256) antibody P00073.
Key=small_image_label, value=Immunofluorescence staining of methanol-fixed MCF7 cells using FKHR(Phospho-Ser256) antibody P00073.
Key=thumbnail_label, value=Immunofluorescence staining of methanol-fixed MCF7 cells using FKHR(Phospho-Ser256) antibody P00073.
Key=msrp_display_actual_price_type, value=
Key=gift_message_available, value=No
Key=size, value=100 µl
Key=gene_full_name, value=Forkhead box protein O1
Key=uniprot_id, value=Q12778
Key=applications, value=IF, IHC, WB
Key=reactivity, value=Human, Mouse, Rat
Key=form, value=Liquid
Key=concentration, value=1 mg/ml
Key=cross_reactivity, value=No cross reactivity with other proteins.
Key=predicted_reactivity, value=Bovine, Chicken, Xenopus Laevis, Xenopus Tropicalis, Zebrafish
Key=source_company, value=SabBiotech 11115; Cost: 159.5
Key=isotype, value=IgG
Key=molecular_weight, value=69662 MW
Key=sample_size_available, value=5ul, free with shipping charge
Key=related_product_skus, value=M00073,PB9195
Key=product_category, value=Primary Antibodies
Key=research_category, value=Apoptosis, Associated Proteins, Cancer, Cell Biology, Co-Activators/Co-Repressors, Domain Families, Epigenetics and Nuclear Signaling, Forkhead Box, Hypoxia, Intracellular, Metabolism, Metabolism Processes, Nuclear Receptors, Nuclear Signaling Pathways, Oncoproteins, Oncoproteins/Suppressors, Pathways and Processes, Transcription, Transcription Factors
Key=clonality, value=Polyclonal
Key=swatch_image, value=/antibody/ek0524-2-ELISA-human-timp-4-picokine-elisa-kit.jpg
Key=rating_value, value=99.4
Key=review_count, value=26
Key=mp_exclude_sitemap, value=No
Key=yoast_facebook_image, value=/m/0/m04858-lalba-primary-antibodies-if-testing-1.jpg
Key=yoast_twitter_image, value=/m/0/m04858-lalba-primary-antibodies-if-testing-1.jpg
Key=cj_related_products, value=Alveolar Rhabdomyosarcoma:EKC2317^Rhabdomyosarcoma, Embryonal:PB9195|A00073-1|A00073S256|A01809-2
Key=description, value=Boster Bio Anti-Phospho-FKHR (Ser256) FOXO1 Antibody (Catalog # P00073). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Key=short_description, value=Boster Bio Anti-Phospho-FKHR (Ser256) FOXO1 Antibody (Catalog # P00073). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Key=meta_keyword, value=Anti-Phospho-FKHR (Ser256) FOXO1 Antibody
Key=contents, value=Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Key=background, value=Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts syngernistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells.
Key=gene_name, value=FOXO1
Key=synonyms, value=Forkhead box protein O1;Forkhead box protein O1A;Forkhead in rhabdomyosarcoma;FOXO1;FKHR, FOXO1A;
Key=application_details, value=Predicted MW: 78-82 kd
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:50-1:200
Immunofluorescence: 1:100-1:500
Key=subcellular_localization, value= Cytoplasm . Nucleus . Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells. In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway. Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation. Phosphorylation by NLK results in nuclear export. Translocates to the nucleus upon oxidative stress-induced phosphorylation at Ser-212 by STK4/MST1. SGK1-mediated phosphorylation also results in nuclear translocation. Retained in the nucleus under stress stimuli including oxidative stress, nutrient deprivation or nitric oxide. Retained in the nucleus on methylation. .
Key=tissue_specificity, value= Ubiquitous. .
Key=application_notes, value=This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. Less than 0.2% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for dual phosphorylated pS423 and pS425 of Smad3. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested.
Key=competitor_equivalent_skus, value=sc 101154
Key=immunogen, value=A synthesized peptide derived from human FOXO1A around the phosphorylation site of Ser256.
Key=purification, value=The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Key=storage, value=Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Key=protein_function, value= Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'- TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC and PCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose tissue. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide- damaged DNA in beta-cells. Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner. .
Key=options
Key=media_gallery
Key=extension_attributes
Key=tier_price
Key=tier_price_changed
Key=category_ids
Key=quantity_and_stock_status
Key=is_salable
Key=website_ids
Key=request_path
Key=rating_summary
Key=_cache_instance_store_filter
Key=media_gallery_images