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Product Info Summary
| SKU: | P00073 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-FKHR (Ser256) FOXO1 Antibody
SKU/Catalog Number
P00073
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-FKHR (Ser256) FOXO1 Antibody (Catalog # P00073). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-FKHR (Ser256) FOXO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00073)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
A synthesized peptide derived from human FOXO1A around the phosphorylation site of Ser256.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P00073 is reactive to FOXO1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
69662 MW
Background of FOXO1
Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts syngernistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00073 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 78-82 kd
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:50-1:200
Immunofluorescence: 1:100-1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from 293 cells untreated or treated with serum using FKHR(Phospho-Ser256) antibody P00073.
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Immunofluorescence staining of methanol-fixed MCF7 cells using FKHR(Phospho-Ser256) antibody P00073.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHR (Phospho-Ser256) antibody P00073 (left) or the same antibody preincubated with blocking peptide (right).
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Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with LY2904 using FKHR (Phospho-Ser256) antibody P00073.
Protein Target Info & Infographic
Gene/Protein Information For FOXO1 (Source: Uniprot.org, NCBI)
Gene Name
FOXO1
Full Name
Forkhead box protein O1
Weight
69662 MW
Alternative Names
Forkhead box protein O1;Forkhead box protein O1A;Forkhead in rhabdomyosarcoma;FOXO1;FKHR, FOXO1A; FOXO1 FKH1, FKHRA, FOXO1 forkhead box O1 forkhead box protein O1|forkhead box protein O1A|forkhead, Drosophila, homolog of, in rhabdomyosarcoma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOXO1, check out the FOXO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOXO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-FKHR (Ser256) FOXO1 Antibody (P00073)
Hello CJ!
P00073 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Prolonged overnutrition with fructose or fat induces metabolic derangements in rats by disrupting the crosstalk between the hypothalamus and periphery: Possible amelioration with fenofibrate
Dong P, Mai Y, Zhang Z, Mi L, Wu G, Chu G, Yang G, Sun S. Acta Biochim Biophys Sin (Shanghai). 2014 Jul;46(7):565-71. Doi: 10.1093/Abbs/Gmu043. Epub 2014 May 26. Mir-15A/B Promote Adipogenesis In Porcine Pre-Adipocyte Via Repressing Foxo1.
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Phospho-FKHR (Ser256) FOXO1 Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for female gonad using anti-Phospho-FKHR (Ser256) antibody P00073. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-19
Question
Our team were well pleased with the WB result of your anti-Phospho-FKHR (Ser256) antibody. However we have seen positive staining in cervix carcinoma cytoplasm. using this antibody. Is that expected? Could you tell me where is FOXO1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
According to literature, cervix carcinoma does express FOXO1. Generally FOXO1 expresses in cytoplasm. Regarding which tissues have FOXO1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231, 23186163
Lymph, and Placenta, Pubmed ID: 15489334
Rhabdomyosarcoma, Pubmed ID: 9479491
Boster Scientific Support
Answered: 2020-03-06
Question
Does anti-Phospho-FKHR (Ser256) antibody P00073 work for WB with female gonad?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
According to the expression profile of female gonad, FOXO1 is highly expressed in female gonad. So, it is likely that anti-Phospho-FKHR (Ser256) antibody P00073 will work for WB with female gonad.
Boster Scientific Support
Answered: 2020-02-07
Question
Does P00073 anti-Phospho-FKHR (Ser256) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
As indicated on the product datasheet, P00073 anti-Phospho-FKHR (Ser256) antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-05
Question
Do you have a BSA free version of anti-Phospho-FKHR (Ser256) antibody P00073 available?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-FKHR (Ser256) antibody P00073 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-07
Question
We are currently using anti-Phospho-FKHR (Ser256) antibody P00073 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
The anti-Phospho-FKHR (Ser256) antibody (P00073) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-25
Question
Is this P00073 anti-Phospho-FKHR (Ser256) antibody reactive to the isotypes of FOXO1?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
The immunogen of P00073 anti-Phospho-FKHR (Ser256) antibody is Peptide sequence around phosphorylation site of serine 256 (A-A-S(p)-M-D) derived from Human FKHR. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-19
Question
Is a blocking peptide available for product anti-Phospho-FKHR (Ser256) antibody (P00073)?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
We do provide the blocking peptide for product anti-Phospho-FKHR (Ser256) antibody (P00073). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-11
Question
We have observed staining in rat lymph placenta. Do you have any suggestions? Is anti-Phospho-FKHR (Ser256) antibody supposed to stain lymph placenta positively?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
According to literature lymph placenta does express FOXO1. According to Uniprot.org, FOXO1 is expressed in female gonad, rhabdomyosarcoma, lymph placenta, cervix carcinoma, among other tissues. Regarding which tissues have FOXO1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231, 23186163
Lymph, and Placenta, Pubmed ID: 15489334
Rhabdomyosarcoma, Pubmed ID: 9479491
Boster Scientific Support
Answered: 2019-06-10
Question
We purchased anti-Phospho-FKHR (Ser256) antibody for IHC on cervix carcinoma a few years ago. I am using rat, and We are going to use the antibody for IF next. Our lab want to know about examining cervix carcinoma as well as female gonad in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2018-12-28
Answer
I looked at the website and datasheets of our anti-Phospho-FKHR (Ser256) antibody and it appears that P00073 has been validated on rat in both IHC and IF. Thus P00073 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-12-28
Question
I have a question about product P00073, anti-Phospho-FKHR (Ser256) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-04-30
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00073 anti-Phospho-FKHR (Ser256) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-04-30
Question
I would like using your anti-Phospho-FKHR (Ser256) antibody for regulation of foxo transcriptional activity by acetylation studies. Has this antibody been tested with western blotting on 293 cells? We would like to see some validation images before ordering.
T. Banerjee
Verified customer
Asked: 2018-03-12
Answer
We appreciate your inquiry. This P00073 anti-Phospho-FKHR (Ser256) antibody is tested on 293 cells, mcf7 cells, breast carcinoma tissue, mef cells. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-03-12
Question
We want to test anti-Phospho-FKHR (Ser256) antibody P00073 on human female gonad for research purposes, then I may be interested in using anti-Phospho-FKHR (Ser256) antibody P00073 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Jha
Verified customer
Asked: 2017-08-17
Answer
The products we sell, including anti-Phospho-FKHR (Ser256) antibody P00073, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-17
Question
I was wanting to use your anti-Phospho-FKHR (Ser256) antibody for WB for human female gonad on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human female gonad identification?
Verified Customer
Verified customer
Asked: 2017-08-01
Answer
As indicated on the product datasheet, P00073 anti-Phospho-FKHR (Ser256) antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human female gonad in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-01
Question
I see that the anti-Phospho-FKHR (Ser256) antibody P00073 works with WB, what is the protocol used to produce the result images on the product page?
H. Moore
Verified customer
Asked: 2016-07-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-07-05
Question
I have attached the WB image, lot number and protocol we used for female gonad using anti-Phospho-FKHR (Ser256) antibody P00073. Please let me know if you require anything else.
E. Wu
Verified customer
Asked: 2013-09-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-09-26
