Ephrin type-A receptor 2 Antibodies

Ephrin type-A receptor 2 (EPHA2)

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands living on adjacent cells, resulting in contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is known as forward signaling while the signaling pathway downstream of the ephrin ligand is known as reverse signaling.

Activated from the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway.

Gene Information

Human
Gene Name:	EPHA2
Uniprot:	P29317
Entrez:	1969

Family

Belongs to:
protein kinase superfamily

Alternative Names

ARCC2; EC 2.7.10; EC 2.7.10.1; Eck; ECKepithelial cell receptor protein tyrosine kinase; EPH receptor A2; EphA2; ephrin type-A receptor 2; Epithelial cell kinase; Myk2; Sek2; soluble EPHA2 variant 1; Tyrosine-protein kinase receptor ECK

Molecular Weight

Mass (kDA):
108.266 kDA

Genomic Context

Human
Location:	1p36.13
Sequence:	1; NC_000001.11 (16124337..16156104, complement)

Tissue Specificity

Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g. skin, intestine, lung, and ovary.

Subcellular Localizations

Cell membrane; Single-pass type I membrane protein. Cell projection, ruffle membrane; Single-pass type I membrane protein. Cell projection, lamellipodium membrane; Single-pass type I membrane protein. Cell junction, focal adhesion. Present at regions of cell-cell contacts but also at the leading edge of migrating cells (PubMed:19573808, PubMed:20861311). Relocates from the plasma membrane to the cytoplasmic and perinuclear regions in cancer cells (PubMed:18794797).

Diseases

• Neoplasms
• Malignant Neoplasms
• Carcinoma
• Neoplasm Metastasis
• Tissue Adhesions
• Tumor Angiogenesis
• Pathologic Neovascularization
• Cell Invasion
• Malignant Paraganglionic Neoplasm
• Mammary Neoplasms
• Malignant Neoplasm Of Breast
• Cell Transformation, Neoplastic

• Melanoma
• Tumor Progression
• Malignant Neoplasm Of Prostate
• Neoplasm Invasiveness
• Adenocarcinoma
• Malignant Neoplasm Of Ovary
• Ovarian Neoplasm

Interacting Proteins

• CTNNB1
• EFNA1
• EFNA5
• EPHA7
• HSP90AB1

• PTK2
• PTPN11

Pathways

• Angiogenesis
• Cell Migration
• Cell Adhesion
• Cell Proliferation
• Cell Growth
• Pathogenesis
• Localization
• Axon Guidance
• Oncogenesis
• Gene Silencing
• Cell Cycle
• Endocytosis
• Rna Interference

• Cell Motility
• Endothelial Cell Migration
• Transport
• Cell Cycle Arrest
• Cell-cell Adhesion
• Tube Formation
• Translation

PTMs

• Phosphorylation
• Dephosphorylation
• Cleavage
• Methylation

• Glycosylation
• Ubiquitination
• Carboxylation
• Ribosylation

Research Areas

• Cancer
• Protein Kinase

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/EPHA2

Boster Biomarker - The Best Uses Of The EPHA2 Marker

Are you searching for a boster biomarker that will permit EPHA2 antibody analysis? You've come to the right place If so. In this article, you'll learn about EPHA2 antibodies and the ACTA1 gene. This marker is often found in blood, muscle and brain tissue. Learn more about its benefits, and how scientists can utilize it to help in their research.

Antibodies to Ephrin type A receptor 2

The function and structure of excitatory synapses is dependent on the Ephrin type A receptors. These antibodies are polyclonal or monoclonal and react with a variety of animal samples. Boster Bio produces these antibodies using rabbit and mouse. They can be used to generate bidirectional signals between neighboring cells in both forward and reverse directions. They are therefore useful tools for researchers who are studying the function of these receptors.

The human protein Ephrin type-A receptors are transmembrane proteins. They mediate various processes in the nervous system and development stages. Ephrins can be classified into two groups based on their extracellular domain sequences: Ephrin-B receptors and ephrin receptors. Ephrin-A receptors are anchored to the cell membrane through a glycosylphosphatidylinositol linkage.

Anti-Actin ACTA1 antibody

Boster Bio Anti Actin ACTa1 antibody is part of Picoband(tm). It was tested for use IHC and WB applications. It can be stored at temperatures of -20°C and is suitable for use in immunohistochemistry (IHC). This antibody reacts with Human, Mouse, and Rat Actin. For use in cellular assays, Boster Bio Anti-Actin ACTa1 antibody is suggested.

The ACTA1 gene encodes a highly conserved proteins that bind actin, a component in every cytoskeletal structure. While the amount of actin is different depending on the cell's differentiation, they share a ninety percent sequence homology. The synthetic peptide used generate antibodies matches the C-terminal 11 human actin residues. Every antibody is validated by Boster Bio against known positive and negative samples.

ACTA1/Alpha peptide antibodies are produced by rabbits immunized with KLH conjugated synthetic peptides. The purified antibodies are purified by the protein A column followed by the affinity purification of peptides. The antibody recognizes actins, which are widely expressed in the eukaryotic cells. The antigen is provided in PBS that contains 0.09 percent sodium azide.

ACTA1 gene

The EPHA2 marker has many uses in biological research. EphA2 monoclonal antibodies made by Boster Bio have been tested in several methods including ELISA, ICC, and WB. The antibody is able to react with mouse and human cells in both the presence and absence of complement. It can also be useful for immunofluorescence research. Here are some of the best uses of EphA2 markers.