Product Info Summary
SKU: | A01357S52-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-Cytokeratin 18 (S52) KRT18 Antibody
SKU/Catalog Number
A01357S52-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Cytokeratin 18 (S52) KRT18 Antibody catalog # A01357S52-1. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Cytokeratin 18 (S52) KRT18 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01357S52-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Keratin 18 around the phosphorylation site of Ser52. AA range:21-70
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01357S52-1 is reactive to KRT18 in Human
Reconstitution
Calculated molecular weight
48058 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01357S52-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of 3T3 293T using p-Cytokeratin 18 (S52) antibody. antibody was diluted at 1:500
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Immunohistochemical analysis of paraffin-embedded Human brain. antibody was diluted at 1:100 (4° overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Click image to see more details
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Keratin 18 (Phospho-Ser52) antibody
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Immunofluorescence analysis of HeLa cells, using Keratin 18 (Phospho-Ser52) antibody. The picture on the right is blocked with the phospho peptide.
Click image to see more details
Western blot analysis of lysates from HepG2 cells, using Keratin 18 (Phospho-Ser52) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For KRT18 (Source: Uniprot.org, NCBI)
Gene Name
KRT18
Full Name
Keratin, type I cytoskeletal 18
Weight
48058 MW
Superfamily
intermediate filament family
Alternative Names
Keratin, type I cytoskeletal 18;Cell proliferation-inducing gene 46 protein;Cytokeratin-18;CK-18;Keratin-18;K18;KRT18;CYK18;PIG46; KRT18 CK-18, CYK18, K18 keratin 18 keratin, type I cytoskeletal 18|cell proliferation-inducing gene 46 protein|cytokeratin 18|keratin 18, type I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT18, check out the KRT18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Cytokeratin 18 (S52) KRT18 Antibody (A01357S52-1)
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