KRT18 Antibodies

Keratin, type I cytoskeletal 18 (KRT18)

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization.

Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Gene Information

Human
Gene Name:	KRT18
Uniprot:	P05783
Entrez:	3875

Family

Belongs to:
intermediate filament family

Alternative Names

Cell proliferation-inducing gene 46 protein; cell proliferation-inducing protein 46; CK-18; CYK18; Cytokeratin 18; cytokeratin-18; K18; Keratin 18; keratin, type I cytoskeletal 18; keratin-18; KRT18

Molecular Weight

Mass (kDA):
48.058 kDA

Genomic Context

Human
Location:	12q13.13
Sequence:	12; NC_000012.12 (52948871..52952906)

Tissue Specificity

Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

Subcellular Localizations

Cytoplasm, perinuclear region. Nucleus, nucleolus.

Diseases

• Neoplasms
• Malignant Neoplasms
• Liver Diseases
• Carcinoma
• Neoplasm Metastasis
• Malignant Neoplasm Of Breast
• Fibrosis
• Mammary Neoplasms
• Liver Cirrhosis
• Tissue Adhesions
• Neoplasm Invasiveness
• Steatohepatitis
• Adenocarcinoma

• Liver Carcinoma
• Malignant Paraganglionic Neoplasm
• Fatty Liver
• Liver Neoplasms
• Nonalcoholic Steatohepatitis
• Stomach Neoplasms
• Hepatitis

Interacting Proteins

• BECN1
• DNAJB6
• GOLGA2
• HAUS1
• HGS

• IKBKG
• KRT6C
• KRT8
• LILRB3
• MACROD1

• NEFL
• NME7
• SYCE1
• TRADD
• TSG101

Pathways

• Cell Death
• Pathogenesis
• Cell Proliferation
• Cell Cycle
• Localization
• Cell Differentiation
• Secretion
• Hyperphosphorylation
• Cell Adhesion
• Hatching
• Transport
• Proteolysis
• Blastocyst Formation

• Reverse Transcription
• Epithelial Cell Differentiation
• Angiogenesis
• Mammary Gland Morphogenesis
• Gluconeogenesis
• Cell Maturation
• Embryo Development

PTMs

• Phosphorylation
• Cleavage

• Glycosylation
• Oxidation

Research Areas

• Cancer
• Cell Biology
• Cellular Markers
• Phospho-Specific

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/KRT18

Best Uses Of The KRT18 Marker

Boster Bio troubleshooting guides will aid you in optimizing your experiments. These guides contain tricks and tips that will help you make the right decisions and maximize your experiments. Boster Bio's troubleshooting guide can help you pinpoint where you might have problems and the best ways to fix the issue. You can also manage your experiment with the KRT18 marker by setting the proper conditions and using the appropriate controls.

Boster Bio optimization tips

It is crucial to optimize your antibody to the KRT18 marker if you are conducting research on it. Boster offers high affinity primaries that have been validated for this specific antigen , which have been cited extensively for over a quarter of a century. They are made of recombinant proteins and polypeptides that target a specific epitope. Here are a few tips to maximize the efficiency of your antibody as well as increase the reproducibility of your research.

Boster Bio troubleshooting guides

In order to utilize immunohistochemistry (IHC) to identify this marker, you need to know how to perform the steps involved. Boster Bio offers protocols that can assist you in completing the tests in the most efficient way. These guides provide troubleshooting suggestions for common problems, for example, low staining strength, high background, or nonspecific staining. In addition, Boster Bio has assembled a wide range of resources on immunohistochemistry, including protocols and techniques that are critical to the success of the experiment.

Benefits of Boster's KRT18 Marker

The RT-qPCR tests have numerous advantages one of which is the ability to measure the expression of genes more accurately. RT-qPCR permits researchers to compare gene expression levels between two samples such as KRT18KD or control. For the latter sample, the RNA samples were processed in the same way as the RNA-seq studies. The primer sequences used for RT-qPCR can be found in Table 2.

The gene KRT18 regulates expression of many SFs and ASEs. This marker is essential in cancer research. KRT18 can be found in various types of tumors, including human cancer cells , and inflammatory diseases. By using this marker, researchers can pinpoint which cancer cells are affected and track the treatment of cancer patients. In addition to identifying cancerous cells, this RNA-seq test is ideal for cancer researchers.