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Product Info Summary
| SKU: | A00277T387-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-Chk2 (T387) CHEK2 Antibody
SKU/Catalog Number
A00277T387-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Chk2 (T387) CHEK2 Antibody catalog # A00277T387-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Chk2 (T387) CHEK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00277T387-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Chk2 around the phosphorylation site of Thr387. AA range:361-410
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00277T387-1 is reactive to CHEK2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
60915 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00277T387-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Validation Images & Assay Conditions
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Western Blot analysis of Hela cells using Phospho-Chk2 (T387) polyclonal antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Chk2 (Phospho-Thr387) antibody
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Chk2 (Phospho-Thr387) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from Jurkat cells, using Chk2 (Phospho-Thr387) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For CHEK2 (Source: Uniprot.org, NCBI)
Gene Name
CHEK2
Full Name
Serine/threonine-protein kinase Chk2
Weight
60915 MW
Superfamily
protein kinase superfamily
Alternative Names
Serine/threonine-protein kinase Chk2;2.7.11.1;CHK2 checkpoint homolog;Cds1 homolog;Hucds1;hCds1;Checkpoint kinase 2;CHEK2;CDS1, CHK2, RAD53; CHEK2 CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1 checkpoint kinase 2 serine/threonine-protein kinase Chk2|CHK2 checkpoint homolog|cds1 homolog|checkpoint-like protein CHK2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHEK2, check out the CHEK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHEK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Chk2 (T387) CHEK2 Antibody (A00277T387-1)
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