Anti-CHEK2 Antibody (N-term)

Product Name

Anti-CHEK2 Antibody (N-term)

View all Chk2 Antibodies

SKU/Catalog Number

A00277-1

Size

80 µl

Form

Liquid

Description

Boster Bio Anti-CHEK2 Antibody (N-term) (Catalog # A00277-1). Tested in WB, IF, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-CHEK2 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00277-1)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This CHEK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-141 amino acids from the N-terminal region of human CHEK2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00277-1 is reactive to CHEK2 in Human

Applications

A00277-1 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

60915 Da

Background of Chk2

CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:10-1:50
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:10-1:50

Validation Images & Assay Conditions

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Confocal immunofluorescent analysis of CHEK2 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

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CHEK2 Antibody (N-term) western blot analysis in K562,NCI-H460 cell line lysates (35ug/lane).This demonstrates the CHEK2 antibody detected the CHEK2 protein (arrow).

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All lanes : Anti-CHEK2 Antibody (N-term) at 1:1000 dilution
Lane 1: HCT116 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 61 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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CHEK2 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CHEK2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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CHEK2 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Gene/Protein Information For CHEK2 (Source: Uniprot.org, NCBI)

Alternative Names

CDS1; CHEK2; CHK2 checkpoint homolog (S. pombe); Chk2; EC 2.7.11; EC 2.7.11.1; HuCds1; LFS2; PP1425; Rad53; S.pombe) homolog CHEK2 CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1 checkpoint kinase 2 serine/threonine-protein kinase Chk2|CHK2 checkpoint homolog|cds1 homolog|checkpoint-like protein CHK2

For more info on CHEK2, check out the CHEK2 Infographic

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In this infographic, you will see the following information for CHEK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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